基于SRAP的锥栗主栽农家品种遗传多样性分析

摘要

利用从256对中筛选出的10对相关序列扩增多态性(SRAP)引物组合对福建省建瓯市的17个锥栗主栽农家品种进行了遗传多样性分析.结果表明:不同农家品种遗传多样性丰富,10对引物组合共扩增出了200个条带,多态性带数(NPB)183个,占总条带数的91.5%,引物组合em2me8和em5me9的多态性条带百分比(PPB)均为100%;遗传相似系数变化范围为0.5082~0.7978,平均为0.6530,大尖嘴(DJZ)和中尖嘴(ZJZ)的亲缘关系最近,DJZ与双峰子(SFZ)的亲缘关系最远.在遗传相似系数为0.6557时,17个锥栗主栽农家品种可分为3大类:第I类包括3个农家品种,为处暑红(CSH)、SFZ、 乌壳长芒(WKCM);第II类包括11个农家品种,为大苞榛(DBZ)、 牛角仔(晚熟)(NJZWS)、 乌榛(WZ)、 铁锥(TZ)、 牛角仔(中熟)(NJZZS)、 欧宁仔(ONZ)、 红仔榛(HZZ)、 黄榛(HZ)、 油桐仔(YTZ)、 白露仔(BLZ)和油榛(YZ);第III类包括3个农家品种,为DJZ、ZJZ和小尖嘴(XJZ).引物组合em2me8构建了锥栗农家品种的DNA指纹图谱.为锥栗杂交育种和遗传作图的亲本选配提供参考.%In this paper, 10 primer combinations screened from 256 of sequence-related amplified polymorphism ( SRAP ) were used to analyze genetic diversity of 17 cultivars of Castanea henryi which grown in Jian′ou City of Fujian Province. The results showed high genetic diversity in these cultivars since 10 primer combinations obtained 200 bands containing the 183 polymorphic bands which took 91.5% proportion. The primer combinations em2me8 and em5me9 showed 100% polymorphism rate. The genetic similarity coefficient was between 0. 5082 and 0. 7978, and the average was 0. 6530, DJZ and ZJZ have the closest genetic relationship while DJZ and SFZ have the farthest genetic relationship. As the dendrogram by SRAP marker, at the genetic similarity coefficient of 0.6557, 17 C. henryi cultivars were divided into 3 classes:the first class had 3 cultivars ( CSH, SFZ and WKCM) , the second class contained 11 cultivars (DBZ, NJZWS, WZ, TZ, NJZZS, ONZ, HZZ, HZ, YTZ, BLZ and YZ), and the rest 3 cultivars (DJZ, ZJZ and XJZ) belonged to the last class. A SRAP fingerprint was built for the 17 C. henryi cultivars by em2me8. The paper provides a basis for selecting parents of crossbreeding and genome mapping.