脂质体包裹TGF-α反义脱氧寡核苷酸抑制人大肠癌细胞的增殖

摘要

目的　研究用转化生长因子α (TGF-α)反义脱氧寡核苷酸(ASODN)对人 大肠癌 细胞株HR8348生长增殖的作用，探讨大肠癌治疗的新途径. 方法　采用人 工合成的TGF-α反 义及无关对照ODN经阳离子脂质体包裹后转染人大肠癌HR8348细胞. 采用MTT法，［3H］- TdR掺 入实验，细胞周期分析，裸鼠体内接种等方法测定瘤细胞体内外增殖的变化. 结果 　经TGF- α反义ODN处理的HR8348细胞与对照组HR8348细胞相比，体外增殖速度减慢（细胞增殖抑制 率达71%）,DNA合成受抑,裸鼠体内成瘤率下降（25% vs 100%）. 结论　TGF-α反义ODN能有效抑制大肠癌细胞的体内外生长增殖，可用于实验性肿瘤基因治疗 研究.%AIM　To investigate the effects of TGF-α antisense oligodeoxynucleotides (ASODN) on human rectal carcinoma cell line HR8348. METHODS　Cationic liposome and lipofectamine were selected as a delivery vector. HR8348 cells were treated wit h ASODN in vitro. HR8348 cells with or without pretreatment were inoculated into nude mice. RESULTS　Proliferation and DNA synthesis of HR8 348 cells treate d with ASODN were greatly inhibited and tumorigenic rate was greatly reduced.CONCLUSION　TGF-α ASODN could inhibit the proliferation of huma n colon cancer cell line in vitro as well as in vivo. The results implicate the potential va lue in colorectal cancer gene therapy.