目的：应用傅里叶显微红外光谱（Fourier transform infrared microspectroscopy，FTIR－MSP）技术分析高温死亡大鼠肾的化学基团变化，为高温死亡鉴定提供新的途径与方法。方法建立不同死亡原因动物模型并分组，包括高温死亡组、脑干损伤死亡组、失血性休克死亡组、窒息死亡组，大鼠死亡后立即提取其肾组织，使用FTIR－MSP技术采集肾小球区域的红外光谱。结果高温死亡组大鼠肾小球组织在3290、3070、2850、1540及1396 cm－1处吸收峰强度增高（P<0．05），A1650/A3290与A1650/A1540的比值降低（P<0．05）。结论应用FTIR－MSP技术分析肾组织化学基团变化可作为高温死亡鉴定的辅助诊断方法。%Objective T o observe the chem ical groups changing in rat kidney w ith regard to fatal hyper-therm ia by Fourier transform infrared m icrospectroscopy (FT IR-M SP ) and to provide a new m ethod to diagnose fatal hypertherm ia. Methods R ats w ere sacrificed by hypertherm ia, brainstem injury, m assive hem orrhage and asphyxiation and divided into groups. T he renal sam ples w ere dissected im m ediately af-ter death. T he data of infrared spectroscopy in glom erulus w ere m easured by FT IR-M SP. Results T he absorbances of 3 290, 3 070, 2 850, 1 540 and 1 396 cm -1 significantly increased (P<0.05),and the ratios of A1650/A3290 and A1650/A1540 significantly decreased (P<0.05) in group of hypertherm ia. Conclusion FTIR-M SP can analyze the changes of chem ical groups of kidney as an auxiliary diagnosis for discrim inating hyper-therm ia w ith other causes of death.
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