Objective To investigate the performance of SiFaTM 23 Plex Kit (beta version) and its population genetics of STR loci in Han population.Methods Genotyping was performed for 1 000 unrelated healthy Han individuals by the kit.The efficiency of the kit was tested,and the frequency data and population genetics parameter information of STR loci were analysed statistically.Results The minimum amplification system could be 6.25 μL.In 25 μL standard reaction system,a satisfied genotyping profiles could be obtained with the DNA content as low as 125 pg.Among the 1 000 individuals,267 alleles were detected by 21 autosomal STR loci of the kit,which conformed to Hardy-Weinberg equilibrium.Fifteen and eleven alleles were observed at the newly added STR loci D1S1656 and D10S1248,respectively,which showed a high polymorphism information content.Conclusion SiFaTM 23 Plex Kit (beta version) is excellent in testing blood samples.Its accuracy,repeatability and sensitivity can satisfy the need of forensic practice,which makes it be applied to forensic-related case work and DNA database establishment.%目的 评估SiFaTM 23 Plex试剂盒(提取测试版)的性能及其所包含的STR基因座在汉族人群中的群体遗传学信息.方法 应用试剂盒对1 000名汉族健康无关个体进行分型检测,检测试剂盒有效性并统计分析STR基因座的频率数据及群体遗传学参数信息. 结果 该试剂盒最小扩增体系可以为6.25 μL,标准体系25 μL中DNA含量低至125 pg可获取理想分型图谱.试剂盒中包含的21个常染色体STR基因座在1 000人份中共检测到267个等位基因,均符合Hardy-Weinberg平衡.新增加的两个STR基因座D1S1656和D10S1248分别检测到15和11个等位基因,多态信息含量丰富. 结论 SiFaTM 23 Plex试剂盒(提取测试版)对于血液样本检测性能优越,准确性、重复性、灵敏度均可满足法医学检案需求,可以应用于法医学实际检案及DNA建库工作.
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