直接扩增法用于重大灾难事故中软骨的个体识别

摘要

Objective T o explore the effectiveness of direct am plification for the ST R analysis of carti-lage, and to accelerate the effectiveness of disaster victim identification. Methods E ighty-eight cartilage sam ples w ere directly am plified by Pow erPlex誖21 kit, and the results of genotyping w ere com pared w ith that obtained by the m agnetic beads m ethod. Results In 88 cartilage sam ples, the ST R genotypes w ere successfully detected from 84 sam ples by direct am plification and m agnetic beads m ethod, and both the results of genotyping by tw o m ethod w ere consistent. Conclusion D irect am plification w ith Pow er-Plex誖21 kit can be used for ST R genotyping of cartilages. T his m ethod is operated easily and prom ptly, w hich has a potential application in the individual identification of m ass disasters.%目的 探讨直接扩增法对软骨STR检验的有效性,以提高灾难受害者身源鉴定工作效率.方法采用PowerPlex誖21试剂盒对88份软骨进行直接扩增法检验,同步进行磁珠法比较分型结果.结果88份软骨检材中,直接扩增法与磁珠法均成功检出84份检材的STR基因型,两者分型结果完全一致.结论PowerPlex誖21试剂盒直接扩增法可以应用于软骨STR分型检验,且操作简便、快速,在重大灾难事故身源鉴定中具有很好的应用前景.