The objective of this study is to investigate the biofilm formation of four kinds of different bacteria in vitro under different culture conditions and the effect of sub-MIC disinfectant on biofilm formation. For thisbiofilm formation were tested by microtiter-plate method under the different nutrient conditionstemperature and pHas well as different concentration of quaternary ammonium compoundsthe results demonstrated that biofilm formation was identified widely among food pathogens. Furthermorehigh temperatureslow concentration of glucose and sodium chloride increased the production of biofilm. From the above resultsa conclusion could be made: Biofilm formation was influenced by many factors. Control of the production of biofilm was important for food safety in the food processing.%研究4种细菌在体外不同培养条件下形成生物膜的能力,并探索了亚致死质量浓度消毒剂对细菌生物成膜的影响.在不同营养物质浓度和培养条件下以及不同质量浓度的季铵盐下,以微孔板法检测菌体成膜性.高温、低浓度的葡萄糖和NaCl促进菌体形成生物膜,高质量浓度葡萄糖和NaC1、较高的培养温度,成膜受到抑制.菌体成膜受到多种因素的影响,合理控制食源微生物生物被膜的形成,是促进食品安全和卫生的重要手段.
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