营养型鸡精对拘束应激小鼠免疫功能的改善作用

摘要

作者研究了营养型鸡精对拘束负荷小鼠免疫功能的改善作用.将小鼠随机分为正常对照组,拘束应激组,25 mg/kg RU486(糖皮质激素受体拮抗剂组),1 mL/hg BEC(营养型鸡精)组及2mL/hg BEC组,每组10只.除正常对照组外,其余各组连续灌胃给药5 d后拘束负荷18 h,拘束恢复3 d后取血取脾脏测定相关指标.结果显示:BEC不仅可以明显改善拘束应激时小鼠脾脏的损伤,提高脾淋巴细胞总数,而且能显著降低应激小鼠血浆中皮质酮的含量,抑制相关凋亡因子的基因表达,提高线粒体膜电位,减少细胞色素C的释放.BEC可以通过抑制应激小鼠脾淋巴细胞的凋亡来维护脾淋巴细胞数目和功能,改善应激负荷引起的免疫抑制作用.%This study aims to study the effect of BEC (BRANDS Chicken Essence, BEC) on immune system in restraint stressed mice. Mice were randomly divided into 5 groups with 10 mice each group: normal control group, restraint control group, 25 mg/kg RU486 (GR antagonist)group, 1 mL/100 g BEC and 2 mL/100 g BEC. The treatment groups were orally administrated with BEC or RU486 for consecutive 5 days, while normal control and stress control groups were given the same volume of water. On the 5th day, all mice were restraint for 18 h except normal group. After 3 days recovery, mice were sacrificed. Blood was distilled and spleens were received to prepare lymphocytes for following tests. The results showed that BEC not only could protect the reduced spleen index and spleen lymphocytes, but also could inhibit the elevated content of corticosterone in plasma. RT-PCR analysis showed that BEC also could recover GR mRNA, Bcl2 mRNA and Bax mRNA in spleen lymphocytes nearly to normal. Meantime, the mitochondrion membrane potential (△Ψmt), the content of CytC in cytoplasm or mitochondrion of spleen lymphocytes were also recovered to normal when compared to stressed mice. All of these results indicated that BEC could protect spleen lymphocytes against apoptosis and improve the immune function in restraint stressed mice.