微生物液氮超低温保存研究进展

摘要

Culture collection is a basic research work in microorganism.Cryopreservation, transfer of biological objects to deep cold anabiosis (-196 ℃) , is an efficient method for long term storage of microorganisms.In this review, the parameters important for development of efficient technologies for cryopreservation of lactic acid bacteria, yeast, microalgae and protozoa are addressed.Furthermore, the problems in liquid nitrogen cryopreservation are discussed in this article.%菌种保存是微生物研究中的一项基础工作.在-196℃下活细胞物质代谢和生长几乎完全停止,液氮超低温保存使微生物的长期稳定保存成为可能.作者就影响乳酸菌、酵母菌、微藻,原生动物液氮超低温保存存活率的因素进行了讨论.此外,作者还讨论了液氮超低温保存中还存在的一些问题.