黑暗链霉菌aprF-G基因功能的研究

摘要

In order to block biosynthesis genes apr￥ and aprG of apramycin in Streptomyces tenebrarius Tt-49,recombinant plasmid pFDIO was introduced to S. tenebrarius Tt-49 by conjugation. And double crossover mutant Tt-49 G53 was obtained finally. Preliminary research on physiological characteristics for double crossover mutant Tt-49 G53 was conducted. The results showed that double crossover mutant Tt~49G53 grew more slowly than parent strain, the level of antibiotic production was 13 u/mL, which was 100 times lower than the level of parent strain. The metabolic products were analyzed by TLC. A pre-judgment of the result was that double crossover mutant Tt-49 G53 couldn't produce apramycin. It was supposed that αprF and aprG were not closely related to secondary metabolism of S. tenebrarius only, but also was associated with the growth and development of producing strain.%以研究黑暗链霉菌Tt-49中生物合成基因aprF-G生理功能为目标,利用接合转移法,将同源重组质粒pFD10导入黑暗链霉菌Tt-49,最终获得同源双交换茵株Tt-49G53,并对其生理特性进行考察.结果显示:其生长速度明显比亲株慢,产抗水平比亲株低100倍,仅为13 U/mL.采用薄层层析法对其代谢产物进行分析,未检测到安普霉素.