茯苓菌丝蛋白双向电泳体系的建立及质谱鉴定

摘要

为建立茯苓菌丝体蛋白双向电泳体系并鉴定部分蛋白质,作者采用双向电泳技术对摇瓶培养的茯苓菌丝体蛋白进行分离,并利用MALDI-TOF/TOF对部分蛋白质进行鉴定.结果表明,2-DE图谱获得蛋白质点数较多、清晰、蛋白质点分离较完全且图谱背景清晰,挑取蛋白质点进行了鉴定.本研究确定的双向电泳条件分离蛋白质效果及重复性好,为下一步利用该方法寻找具有重要功能的新茯苓菌丝体蛋白创造了条件.%To establish an efficient 2-DE protocol for analysis of Poria cocos protein and identification of the selected protein spots,the total protein of flask liquid fermented P.cocos was separated by 2-DE and the selected protein spots were analysis by MALDI-TOF/TOF.The results showed an efficient 2-DE of flask liquid fermented P.cocos total protein was established,high quantity protein spots was obtained,most of protein spots had high resolution and clear background,and then the selected protein spots was identified.This study established an useful conduction of 2-DE of P.cocos protein,a high resolution and reproducibility 2-DE image which will be expected to have excellent applications in finding new important functional proteins with proteomics analysis.