β-紫罗兰酮脂质体包封率测定方法研究

摘要

采用薄膜超声法制备β-紫罗兰酮脂质体,通过反透析法分离脂质体和游离物质,并以高效液相色谱法为分析手段对其包封率进行测定.在选定色谱条件下,辅料不干扰测定,β-紫罗兰酮在5～80 mg/L的质量浓度范围内线性关系良好(R2=0.999 8),日内和日间精密度(RSD)均小于3％;透析平衡时间为12h,游离物回收率符合要求(99.21％～101.22％),且透析20 h内无渗漏,方法重现性好;使用此法测得β-紫罗兰酮脂质体的平均包封率为(52.76±1.10)％.反透析法简便,准确,经济,适用于β-紫罗兰酮脂质体包封率的测定.%A method used for determining the entrapment efficiency of beta-ionone in liposomes was established.The liposome was prepared by thin film evaporation.The free beta-ionone was separated from liposomes by reverse dialysis and quantified with HPLC.The results showed that the calibrated curve was linear (R2=0.999 8) within the range of 5～80 mg/L for beta-ionone.Intra-day and inter-day precision (RSD)were both less than 3％.The equilibrium time for the dialysis was 12 h,and the recoveries showed with good reproducibility (99.21％～101.22％),and the leaking percentage in 20 h was negligible.The average entrapment efficiency of beta-ionone liposome was 52.76 ±1.10％.The reverse dialysis method was convenient,accurate and economic,suitable for determining the encapsulation efficiency ofbeta-ionone liposomes.