转基因玉米BT11品系环介导等温扩增（LAMP）检测方法的建立

摘要

Objective To establish a loop-mediated isothermal amplification(LAMP)detection method for BT 11. Methods The primers were designed according to the specific sequence(AY123624.1, AY629236)of BT 11, and then the LAMP reaction system was established. The specificity, sensitivity and stability of the system were analyzed. Results The primers in this research specifically detected the BT 11. The detection sensitivity was up to 0.5%, and the precision of the experimental analysis was proved to be of high stability. Conclusion The results showed that LAMP was a specific, stable, rapid, sensitive and visible way for the detection of BT 11, and it would have broad prospects for application.%　　目的建立转基因玉米BT 11品系的环介导等温扩增(LAMP)检测方法。方法根据转基因玉米BT 11品系特有序列(AY123624.1和AY629236)设计引物，优化建立LAMP反应体系，对该体系进行特异性、灵敏度、稳定性分析。结果本研究设计的引物具有很好的特异性，可特异性检测出转基因玉米BT 11品系；检测灵敏度可达0.5%；经精密度实验分析，该方法的稳定性良好。结论 LAMP方法检测转基因玉米品系BT 11具有特异性高、稳定性好、快速灵敏、过程可视等优点，具有广阔的应用前景。