目的 探究不同温度、过氧化氢酶浓度、反应时间等对牛皮中过氧化氢分解速率的影响规律, 以此指导生产中如何控制狗咬胶中过氧化氢残留量超标的问题.方法 以生产宠物狗咬胶的漂白牛皮为原料, 通过采用不同温度(30~70)℃ 的水和不同浓度(3%~5%)的过氧化氢酶溶液进行处理, 对处理后的过氧化氢残留量进行测定.结果 采用50 ℃温水浸泡漂白牛皮可以较快地去除其中残留的过氧化氢; 或者采用浓度为3%的过氧化氢酶溶液, 40~50 ℃, 浸泡30 min左右可以更快地去除过氧化氢.根据处理条件的不同, 通过试验拟合出方程可以比较准确地计算去除漂白牛皮样品中残留过氧化氢所需要的大体时间.结论 通过热处理或者过氧化氢酶溶液处理, 能够有效降低漂白牛皮中残留的过氧化氢含量, 使最终产品符合要求.%Objective To investigate the effects of different temperatures, catalase concentrations and reaction time on the decomposition rate of hydrogen peroxide in bleached cow skin, in order to guide the production how to control the residual hydrogen peroxide in pet chew. Methods The residues of hydrogen peroxide in bleached cow skin as raw material were tested by different treatments, including 30~70 ℃ warm water treatment and 3%~5% catalase treatment. Results After soaking with 50 ℃ warm water, residual hydrogen peroxide in the bleached cow skin could be quickly removed. In addition, when adopting catalase solution treatment, the optimum enzyme concentration was 3%, at temperature of 40~50 ℃ for about 30 minutes. According to the different treatment conditions, it was more accurate to calculate the general time required to remove the residual hydrogen peroxide in the sample of bleached cow skin. Conclusion It can effectively reduce the residual hydrogen peroxide content in bleached cow skin by heat treatment and catalase solution, which can make the final product meet the requirements.
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