几种提取溶剂对富硒香菇中有机硒含量测定的影响

摘要

目的 研究不同提取溶剂对富硒香菇中的有机硒含量测定的影响.方法 采用纯水、无水乙醇及盐酸(6 mol/L)3种不同提取溶剂对香菇中无机硒进行提取,通过测定总硒和无机硒,两者差减而得到有机硒含量,探讨不同提取体系对有机硒含量测定的影响.结果 用乙醇提取,仅有一半有机硒被提取出,无法准确测定有机硒含量;盐酸(6 mol/L)提取,会导致无机硒测定结果略为偏高且操作较繁琐,不适合香菇中有机硒的测定;直接用水提取,测定结果比较符合香菇中有机硒的含量.直接用水提取方法的测定检出限0.039μg/L,无机硒加标回收率为99.26%~102.72%,精密度测定相对标准偏差小于4.81%.结论 水提取测定无机硒,通过总硒相减得到有机硒含量,对于香菇中有机硒含量测定,该法简单、快速,是的一种较为理想的方法.%Objective To investigate the effect of different extraction solvents on the determination of organic selenium in edible selenium-enriched mushrooms.MethodsSamples were extracted by ethanol, water and HCl (6 mol/L). The organic selenium was figured out by subtract inorganic selenium from total selenium after detection. ResultsThe results showed that only half of the organic selenium could be extracted by ethanol, which might lead to inaccurate measurement. The detected inorganic selenium result was a little higher than the actual value by HCl extraction, which was not suitable for the determination of organic selenium in mushrooms. Water extraction was suitable for organic selenium determination for its result was conformed with the actual value. The detection limit of inorganic selenium was 0.039μg/L. The recoveries of inorganic selenium were 99.26%~102.72%, with the RSD less than 4.81%.ConclusionWater is a perfect solvent for inorganic selenium extraction, which is proved to be a simple, rapid, and suitable method for the detection of organic selenium in selenium-enriched mushrooms.