This study was purposed to investigate the expression of Btk and NFkB in acute myeloid leukemia (AML) cells and its significance. Bone marrow mononuclear cell specimens were taken from 14 AML patients who were in new diagnosis and complete remission respectively, the expressions of Btk and NFkB at mRNA and protein levels were detected by RT-PCR and Western blot, respectively. The results showed that Btk and NFkB expressed in all the samples at RNA and protein levels. At protein level, Btk and NFkB expressions were higher in the cells from newly diagnosed AML patients than that in the cells from patients in complete remission stage (P < 0. 05). It is concluded that Btk and NFkB may play an important role in the development and progression of AML, they may be used as potential therapeutic targets of AML and used in predicting the prognosis.%本研究检测Btk及NFκB在急性髓系白血病(AML)患者白血病细胞中的表达,评价其在AML发生、发展中的作用.选取14例AML初诊及治疗达完全缓解患者的骨髓单个核细胞标本,检测Btk、NFκB在mRNA及蛋白质水平的表达.结果表明:在mRNA及蛋白质水平,AML患者细胞中均有Btk、NFκB的表达.Btk、NFκB蛋白在初发AML患者白血病细胞中表达高,达完全缓解后,其表达下降或检测不到(P＜0.05).结论:Btk、NFκB在AML的发生、发展中可能起重要作用,它们有可能作为AML的潜在治疗靶点及用于预后预测.
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