PDK1对中性粒细胞分化的影响

摘要

Objective:To knock out PDK1 by using Vav-Cre and observe the effects of PDK1 knock out on the ratio,number and differentiation of neutrophil.Methods:The PDK1 expression level of Vav-Cre;PDK1lox/lox mouse in the bone marrow cells was analyzed by RT-PCR.The effect of PDK1 on hematopoietic progenitor was observed by CFU-C assay,and the effect of PDK1 on the ratio and number of neutrophil was detected by flow cytometric analysis.Results:The RNA expression level of bone marrow cells of Vav-Cre;PDK1 lox/lox mouse was dramatically lower than that of the control mouse.The number of functional GMP was lower in Vav-Cre;PDKllox/lox mouse in contrast to controls.The percentage and number of neutrophil were lower,but the percentage of premyelocyte/myelocytes was more than 2 times in Vav-Cre;PDKllox/lox group compared with control groups.Conclusion:PDK1 affects the process of the differention of hematopoietic stem cells to GMP,the neutrophil differention and maturation.%目的:应用Vav-Cre在成体小鼠敲除PDK1并观察PDK1敲除对中性粒细胞比例、数目及分化的影响.方法:应用RT-PCR分析PDK1敲除后骨髓细胞PDK1表达水平;应用CFU-C实验检测PDK1对祖细胞功能的影响;应用流式细胞术分析PDK1对中性粒细胞比例的影响.结果:PDK1敲除后骨髓细胞PDK1表达水平很低;粒系祖细胞及巨噬祖细胞数目减少;中性粒细胞比例数目减少,但幼稚的中性粒细胞比例增多.结论:PDK1敲除影响了造血干细胞向祖细胞分化的过程,以及中性粒细胞的分化成熟过程.