A real-time fluorescent quantitative immuno-polymerase chain reaction(RT-IPCR)assay was developed for the detection of non-dioxinlike polychlorinated biphenyl(PCB)congener in soil samples.Based on the construction of 3,4-dichlorinated biphenyl(IUPAC PCB12)hapten and its immunogen,the specific polyclonal antibodies(pAbs)to PCB12 was obtained and used to develop a direct competitive RT-IPCR assay.Using the optimized assay,a standard curve for PCB12 was prepared.The linear range for the determination of PCB12 was from 10.0to 1.0 × 106fg/mL with a correlation coefficient of 0.98 and a detection limit of 1.53fg/mL.The RT-IPCR assays were tested for their cross-reactivity profiles using four selected congeners and four Aroclor products.The results for the soil samples correlated with the concentrations of PCBs obtained by gas chromatography/mass spectrometry.This highly specific,sensitive,and robust assay can be applied to on-site tests of PCBs and serve as a model for other pollutant immunoassays.
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