中华蜜蜂气味受体基因AcOr170的克隆与序列分析

摘要

本研究采用自行设计的引物对中华蜜蜂Apis cerana cerana雄蜂触角中气味受体基因(Odorant receptors 170)AcOr170的cDNA序列进行了克隆和序列分析,以探寻中华蜜蜂雄蜂气味受体AcOr170基因在近缘种昆虫间的进化差异.结果表明:中华蜜蜂雄蜂气味受体基因AcOr170的cDNA序列总长度为1356 bp,编码区序列长度为1188 bp,共编码396个氨基酸,其分子量为46.272 kDa,等电点8.96,Genbank登录号:KX264359.结构域的分析结果显示,该蛋白具有7tm-6一个保守结构域.经序列比对后发现,Or170的序列在中华蜜蜂、西方蜜蜂和大蜜蜂间的亲缘性很近.%Aprimer for the gene Odour receptor 170 of Apis cerana cerana (AcOr170) was designed to clone its cDNA sequence in drones.The aim was to compare evolutionary differences between the Apis cerana cerana and other honeybee species.The experimental results showed that the overall sequence of AcOr170 was 1356 bp in length.The coding region length was 1188 bp,encoding 396 amino acids.Its molecular weight was 46.272 kDa,isoelectric point was 8.96,and the accession ID of Genbank was KX264359.Structural domain analysis indicated that the encoded protein contained a highly conserved structure of 7tm-6.Sequence alignment showed that genome sequences were highly similar among A.cerana cerana,A.mellifera linnaeus and A.dorsata.