不同薄壳山核桃品种的根际土壤细菌菌群结构及多样性研究

摘要

选择江苏薄壳山核桃主产区波尼(BN)、马汉(MH)、威斯顿(WSD)和肖肖尼(XXN)这4个主要品种,利用Illumina Miseq高通量测序技术研究其根际土壤微生物多样性及群落结构组成.结果显示,4种薄壳山核桃品种间根际土壤细菌群落结构在门、属水平上相似,α多样性指数无显著差异.变形菌门、拟杆菌门和芽单胞菌门均为优势门类,假单胞菌属(1％)和Chitinophagaceae菌群(5％)在4个薄壳山核桃品种的根际土壤中丰度均较高.研究初步阐述了薄壳山核桃根际土壤微生物多样性及群落结构组成,为进一步研究薄壳山核桃的根际效应提供了理论依据.%The Miseq molecular technology was used to explore difference between 4 varieties of Carya illinoensis("BN"、"MH"、"XXN"and"WSD")in community structure and diversity of soil bacteria in rhizosphere.It was found that the 4 varieties did not vary much in community structure and diversity of soil bacteria in rhizosphere at the phylum and genus levels or in α-diversity index.Proteobacteria,Bacteroidetes and Gemmatimonadete were dominant phyla.Pseudomonas(1％)and Chitinophagaceae(5％)were quite high in mean abundance in the rhizosphere of all the four varieties of Carya illinoensis,which might be attributed to the chemotaxis of juglone.This study has tentatively elaborated community struc-ture and diversity of the soil bacteria in rhizosphere of the Carya illinoensis trees and may serve as theoretical basis for fu-ture studies on rhizosphere effect of Carya illinoensis.