一种新型葡聚糖基水凝胶敷料的制备及其性质

摘要

将葡聚糖季铵盐衍生物(Dex-GTMAC)与丁二酸酐(SA)分别按摩尔比1∶1和1∶2反应,得到了不同羧基化程度的葡聚糖基大分子Dex-GTMAC-SA(1∶1)和Dex-GTMAC-SA(1∶2),并将其与合成的2,2'-(乙烯二氧)双(乙胺)甲基丙烯酰胺(DA-MA)小分子单体合成可光照交联的葡聚糖基大分子Dex-GTMAC-SA-DAMA(1∶1)和Dex-GTMAC-SA-DAMA(1∶2),再通过原位光交联方法制备得到新型生物可降解水凝胶敷料.通过测试得到Dex-GTMAC-SA-DAMA(1∶1)和Dex-GTMAC-SA-DAMA(1∶2)水凝胶的溶胀率分别为711.7％和645.0％;采用质量浓度为0.3 mg/mL的胰蛋白酶磷酸盐缓冲液降解这两种水凝胶,5d后水凝胶形态不复存在,变成白色粉末,失重率分别为28.3％和22.5％;通过紫外分光光度法测得载药水凝胶在磷酸盐缓冲液(PBS)中浸泡10 d后基本达到释药平衡,药物累计释放量分别达到68.4％和57.9％;通过琼脂平皿扩散法证实,载药的水凝胶相比于不裁药的水凝胶,前3d的抑菌圈更为明显,显示出更为优良的抗菌性;通过荧光光谱分析法,得到两种水凝胶的表面蛋白吸附量分别为0.0038和0.0017 mg/cm2,具有良好的抗蛋白吸附能力.研究结果表明该水凝胶有望成为一种新型的生物可降解的抗菌水凝胶敷料.%Carboxylated quarternary ammonium-dextran(Dex-GTMAC-SA(1 ∶ 1) and Dex-GTMAC-SA (1 ∶ 2)) were synthesized by the reaction between quarternary ammonium-dextran(Dex-GTMAC) and succinic anhydride(SA) with the molar ratio of 1 ∶ 1 and 1 ∶ 2.The novel hydrogels were prapared by photo crosslinking dextran macromolecular (Dex-GTMAC-SA-DAMA (1 ∶ 1) and Dex-GTMAC-SA-DAMA(1 ∶ 2)) which were synthesized by the reaction between Dex-GTMAC-SA and 2,2'-[1,2-ethanediylbis(oxy)]bis-methacrylamide(DA-MA).The results show that Dex-GTMAC-SA-DAMA(1 ∶1) and Dex-GTMAC-SA-DAMA(1 ∶ 2) hydrogels swelling rate are 711.7％ and 645.0％,respectively.After 5 days degradation in the phosphate buffer solution(PBS) of trypsin with the concentration 0.3 mg/mL,the hydrogels turn into white powder and the weight loss rate are 28.3％ and 22.5％,respectively.Chlorhexidine-loaded hydrogels keep a constant release period after 10 days in the phosphate buffer solution(PBS) and finally the cumulative release of hydrogels are 68.4％ and 57.9％ by ultraviolet spectrophotometry.It is proved by agar plate diffusion method that chlorhexidine-loaded hydrogels show more excellent antimicrobial properties than Chlorhexidine-unloaded hydrogels in the first three days.The protein absorption of hydrogels are 0.0038 and 0.0174 mg/cm2 using spectrofluorimetry,suggesting both of them having anti-protein adsorption capacity.It is demonstrated that the hydrogel is expected to be a novel biodegradable and antibacterial wound dressing.