西藏牦牛12SrRNA基因全序列测定及系统进化关系

摘要

以西藏11个地区的牦牛类群共计110头为研究对象,采用PCR方法,首次从耳组织总DNA中扩增出了线粒体12SrRNA基因,并进行了序列测定及分析.结果表明,该基因的长962～965 bp;序列分析表明12SrRNA基因有较高的进化速率,11个地区的牦牛品种同源性相对较高;对11种类群的牦牛及亚洲水牛、非洲水牛、欧洲野牛和家牛四种牛亚科的12SrRNA基因序列建立NJ和ME分子进化树,结果显示帕里牦牛、斯布牦牛、巴青牦牛、丁青牦牛、江达牦牛、工布江达牦牛、康布牦牛与桑日牦牛的亲缘关系最近.%The mitochondrial DNA 12SrRNA of Tibetan yak in 11 populations was sequenced and analyzed by sequencing and bio-information methods.The results showed that the complete sequence of 12SrRNA genefor Tibetan yak was 962 bp～965 bp.The further sequence analysis showed that 12SrRNA gene had a higher evolution rate,they had more homology.It established NJ and ME of 12SrRNA gene phylogenetic tree for 11 kinds of yak and other four bovinaes.