MiR-146a在寻常型银屑病患者外周血单个核细胞中的表达及其临床意义

摘要

Objective:To detect the expression of miR-146a in peripheral blood mononuclear cell ( PBMC) of patients with psoriasis valguris and investigate the correlation between miR-146a and pathogenicity of psoriasis valguris.Methods:The level of miR-146a in the PBMC from 30 psoriasis valguris patients was measured by quantitive revere transcription polymerase chain reac-tion ( RT-PCR) , and its relationship with disease activity index of psoriasis vulgaes patient was in-vestigated.Results:The copies of miR-146a in PBMC of psoriasis vagulars patients was signifi-cantly higher than that in healthy control group ( P<0.05 ) , and there was significant difference between the active stage and the resting stage of psoriasis vulgaris ( P<0.05 ) .The expression of miR-146a has a positive corelation with PASI scores(r=0.62,P<0.05).Conclusion:Elevated miR-146a levels in peripheral blood mononuclear cell ( PBMC) of patients with psoriasis valguris are associated with a high stage of psoriasis vulgaris which indicate that miR-146a probably play an important role in the pathogenesis of psoriasis vulgaris.%目的：检测寻常型银屑病患者外周血单个核细胞（ PBMC）中miR-146a的表达情况，并探讨其与寻常型银屑病发病的相关性。方法：应用实时荧光定量聚合酶链反应检测30例寻常型银屑病患者外周血PBMC中的miR-146a的相对表达量，与30例正常对照组进行比较并对其与寻常型银屑病患者PASI评分行相关性分析。结果：寻常型银屑病患者外周血PBMC中的miR-146a的表达明显高于正常对照组，进行期患者组明显高于静止期患者组，差异均有统计学意义（ P值均＜0.05）。寻常型银屑病患者外周血PBMC中miR-146a的表达与 PASI评分呈正相关性（r＝0.62，P＜0.05）。结论：寻常型银屑病患者外周血PBMC中miR-146a异常高表达，并与寻常型银屑病的分期相关，提示 miR-146a在寻常型银屑病的发病中起重要作用。