从三七茎叶皂苷中分离纯化人参皂苷C-Mx1和Rb3

摘要

The ginsenoside C-Mx1 and Rb3 were isolated and purified from notoginseng leaf ginsenosides.30 g notoginseng leaf ginsenosides were isolated, from which 50%-60% crude C-Mx1 of 4.66 g and Rb1 of 8.58 g were obtained.95% pure C-Mx1 of 0.92 g and Rb3 of 3.1 g were obtained from 4.66 g crude C-Mx1 and 8.58 g crude Rb3.The separation products were C-Mx1 and Rb3 saponins determined by 13C NMR.%利用两步硅胶柱层析法,从三七茎叶皂苷中分离纯化人参皂苷Rb3和C-Mx1单体.30 g三七茎叶皂苷,经分离得到纯度为50%~60%的粗品C-Mx1 4.66 g和Rb3 8.58 g;将C-Mx1和Rb3粗品皂苷进行二次硅胶柱分离,得到纯度为95%的C-Mx1单体0.92 g和Rb3单体3.1 g.以核磁共振法证明了分离产物为C-Mx1和Rb3皂苷.