首页> 中文期刊> 《大连海洋大学学报》 >人工放流4龄中华鲟盐度适应过程中血清生化指标的变化

人工放流4龄中华鲟盐度适应过程中血清生化指标的变化

             

摘要

为研究人工放流4龄中华鲟Acipenser sinensis对长江口盐度的可能适应性,以淡水为对照,测定了在盐度为5、10急性胁迫下,鱼体在6、12、24、48、96 h时各种血液生化指标的含量.结果表明:与对照组相比,4龄中华鲟进入盐度为5的水中后,6h时鱼血清中谷丙转氨酶(ALT)含量显著升高(P<0.05); 12h时,血清中血糖(GLU)、高密度脂蛋白胆固醇(HDL-C)含量显著升高(P<0.05),而ALT含量恢复至对照组水平;24h时,尿素(UREA)含量显著降低(P<0.05),而GLU、HDL-C含量则恢复至对照组水平.4龄中华鲟进入盐度为10的水中后,6h时鱼血清中总蛋白(TP)、ALT含量显著升高(P<0.05);12 h时,血清中TP、GLU、甘油三酯(TG)、HDL-C含量显著升高(P<0.05),而ALT含量则恢复至对照组水平;24h时,血清中胆固醇(CHOL)、HDL-C含量显著降低(P<0.05),而TP、GLU、TG含量则恢复至对照组水平;48、96h时,血清中HDL-C含量显著降低(P<0.05),而CHOL含量恢复至对照组水平.本研究表明,在4龄中华鲟盐度适应过程中,蛋白质可能优先于其他物质被动员而发挥调节作用,糖和脂肪则是机体重要的能量来源.%In order to understand salinity adaptation of Chinese sturgeon Acipenser sinensis, some biochemical parameters were measured in serum of 4-years-old Chinese sturgeon exposed to freshwater ( as the control) and a salinity of 5 and 10 brackish water at 6, 12, 24, 48 h, and 96 h. The results showed that the serum alanine amin-otransferase( ALT) activity was increased significantly in the fish exposed to a salinity of 5 at 6 h, compared with the control(P<0. 05) , and recovered to the control level at 12 h. The glucose (GLU) , and high-density lipopro-tein cholesterol (HDL-C) contents were found to be significantly increased at 12 h(P<0. 05) and recovered to the control level at 24 h. However, urea (UREA) content was significantly decreased at 24 h(P<0. 05). In the fish exposed to a salinity of 10, serum total protein(TP) content and ALT activity at 6 h, TP, GLU, triglyceride(TG), and HDL-C contents at 12 h were significantly increased compared with die control (P<0. 05). Total cholesterol (CHOL) content at 24 h, and HDL-C content at 24, 48 h and 96 h were shown to be significantly decreased(P< 0. 05). ALT activity at 12 h, TP, GLU, and TG contents at 24 h, and CHOL content at 48 h were found to be recovered to the control levels. The findings indicate that serum protein plays a regulatory role with priority of carbohydrate and lipids which function importantly in energy supply during the adaptation to brackish water in 4-years-old Chinese sturgeon.

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