为研究H-FABP基因在兴凯湖野生和养殖翘嘴鲌Culter alburnus 各相同组织中表达的差异, 根据GenBank中已知鲤科鱼类的H-FABP基因序列进行引物设计, 采用RT-PCR和RACE方法克隆出兴凯湖翘嘴鲌心型脂肪酸结合蛋白(H-FABP)的全CDS区序列(402 bp)、 3′UTR区序列(242 bp)和5′UTR区序列(134 bp)共778 bp的cDNA序列.将其提交到GenBank中, 获得基因登陆号为KJ629286, 通过Blast比对发现, 兴凯湖翘嘴鲌H-FABP基因的CDS区与鲤Cyprinus carpio和齐口裂腹鱼Schizothorax prenanti的同源性高达91%; 氨基酸同源性比对显示, 兴凯湖翘嘴鲌H-FABP氨基酸序列与虹鳟Oncorhynchus mykiss的同源性为76%, 与斑马鱼Danio rerio的同源性为85%; 分析野生翘嘴鲌7 个组织(鳃、 心脏、 肠、 肝胰脏、 肌肉、 肾脏、 脾脏)以及养殖翘嘴鲌除心脏之外的6 个相同组织H-FABP基因的表达情况, 结果显示, H-FABP基因在野生组和养殖组肝胰脏中表达量均为最高, 与其他组织的表达量有显著性差异(P<0.05), H-FABP基因在野生组和养殖组各相同组织中的表达量无显著性差异(P>0.05).研究表明, 兴凯湖翘嘴鲌H-FABP基因序列高度保守, 与鲤形目鱼类的同源性更高.%The 778 bp cDNA sequence including whole CDS sequence(402 bp), whole 3′UTR sequence(242 bp)and partial 5′UTR sequence(134 bp)of heart type fatty acid binding protein(H-FABP)in culter Culter alburnus was cloned by RT-PCR and RACE technology to evaluate difference between the amount of expression of H-FABP gene in wild and farmed culter in Xingkai Lake, according to the H-FABP gene sequences of members in Cyprinidae known in Genbank with accession number of KC782835.The Blast showed that there was 91% homology in H-FABP gene CDS area between culter and common carp Cyprinus carpio and prenant's schizothoracin Schizothorax prenanti.The comparison of amino acid homology revealed that the H-FABP gene of the culter showed 76%homology with rainbow trout Oncorhynchus mykiss and 85% homology with zebrafish Danio rerio.The expression of H-FABP gene in 7 tissues(gill, heart, intestine, hepatopancreas, muscle, kidney, spleen)of wild Culter alburnus and in 6 tissues except heart of cultured culter indicated that the maximal mRNA expression of H-FABP gene was observed in hepatopancreas of wild and cultured culter, with significantly different from other tissues(P<0.05).In the same tissue, however, there was no significant difference in mRNA expression level of H-FABP gene in wild and cultured culter(P>0.05).It is concluded that the conservative H-FABP gene in culter had high homology with that in members in Cyprinidae.
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