罗氏 e601电化学发光仪剩余试剂的再利用评价

摘要

Objective To evaluate the recycling value of remaining reagent by Roche e601 electrochemiluminescence ana-lyzer.Methods The precision, accuracy, comparison and correlation analysis were performed between original reagents and recycling reagents for HBsAb、HBcAb、HBeAg、and HBeAb.Results The intra batch precision results of level 1 were 3.43, 3.88,2.04,1.22 respectively,the intra batch precision results of level 2 were 1.22,2.40,1.09,1.66 respectively;the inter batch precision results of level 1 were 5.17,4.55,3.70,3.74 respectively;the inter batch precision results of level 2 were 4.99,3.93,4.16,6.91 respectively.The accuracy result was consistent with the EQA resuthe correlation coefficient of HBsAb was 0.999;the positive rate and negative coincidence rate(%)of HBcAb,HBeAg,HBeAb were 100%.Conclusion The remaining reagents can be combined and recycled by Roche e601, demonstrate good stability and ensure the quality of measurement.%目的：对罗氏e601电化学发光仪剩余试剂再利用进行评估。方法将HBsAb、HBcAb、HBeAg、HBeAb的混合剩余试剂，进行精密度、正确度验证并与新试剂进行比对及相关性分析。结果剩余混合试剂HBsAb、HB-cAb、HBeAg、HBeAb水平1批内精密度分别为3．43、3．88、2．04、1．22，水平2批内精密度分别为1．22、2．40、1．09、1．66；HBsAb、HBcAb、HBeAg、HBeAb水平1批间精密度分别为5．17、4．55、3．70、3．74；水平2批间精密度分别为4．99、3．93、4．16、6．91；正确度本室测定结果与室间质评回报结果均相符、HBsAb相关系数r为0．999；HBcAb、HBeAg、HBeAb两试剂盒的阳性符合率和阴性符合率均为100％。结论罗氏e601电化学发光仪剩余试剂可以混合使用，稳定性良好，能保证检测质量。