紫茉莉提取物对乙醇诱导L-02肝细胞损伤的影响

摘要

Objective:To study the effect of extracts form Mirabilis jalapa on the damaged L-02 human embryo hepatocytes induced by ethanol. Methods: L-02 human embryo hepatocytes in logarithmic phase were chosen and damaged by ethanol at a final concentration of 100 mmol/L. After 8 hours, different concentrations of the extracts form Mirabilis jalapa were added in. After 16 hours of cultivation, MTT method was used to observe inhibition rate of L-02 human embryo hepatocytes damaged by ethanol, and Elisa method was used to detect the activity of glutamic-pyruvic transaminase (ALT), glutamic-oxalacetic transaminase (AST) and superoxide dismutase(SOD), and the content of malonaldehyde(MDA)in nutrient solution. Results:8 mg/mL or higher crude herbal concentrations of Mirabilis jalapa can significantly decrease inhibition rate of ethanol-induced damaged L-02 cells, obviously reduce ALT and AST activity, increase SOD activity and decrease MDA content in the culture for damaged cells. Conclusion:Extracts form Mirabilis jalapa has protective effect on the damaged L-02 cells induced by ethanol.%目的：研究紫茉莉提取物对乙醇体外诱导L-02肝细胞损伤的作用。方法：选取处于对数生长期的L-02肝细胞，以终浓度为100 mmol/L的乙醇诱导损伤8 h后，加入不同浓度的紫茉莉提取物，继续培养16 h后，MTT法检测乙醇对肝细胞的抑制率，酶标法检测培养液中谷丙转氨酶（ALT）、谷草转氨酶（AST）及超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量。结果：8 mg/mL或更高生药浓度的紫茉莉能减小乙醇对L-02肝细胞的抑制率，降低受损细胞培养液中的ALT和AST活性，增强SOD活性，减少MDA含量。结论：紫茉莉提取物对乙醇诱导L-02肝细胞损伤有一定的保护作用。