决明子及炒决明子HPLC化学指纹特征及6种成分的比较研究

摘要

目的:对决明子及炒决明子的化学指纹特征及6种成分进行比较研究.方法:采用高效液相色谱法建立12批决明子及炒决明子未水解样品的化学指纹图谱并对橙黄决明素、芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚6种成分进行分析.结果:12批决明子与决明子对照图谱及炒决明子对照图谱比较,相似度分别为0.94和0.67;12批炒决明子与炒决明子对照图谱及决明子对照图谱比较,相似度分别为0.92和0.62,差异均有统计学意义(P＜0.01).与决明子相比,炒决明子中橙黄决明素等6种成分的含量均增加,其中大黄素、大黄酚及大黄素甲醚差异有统计学意义(P＜0.01或0.05).结论:化学指纹图谱可用于鉴别未水解样品中大黄素、大黄酚和大黄素甲醚可用于决明子炒制前后内在成分含量变化的评价.%Objective:To make a comparative study of the chemical fingerprint features and 6 components of Semen Cassiae and fried Semen Cassiae.Methods:The fingerprints were established by HPLC with 12 batches of Semen Cassiae and fried Semen Cassiae without hydrolysis,and the contents of aurantio-obtusin,aloe-emodine,rhein,emodin,chrysophanol and physcion were comparatively analyzed.Results:When the 12 batches of Semen Cassiae were compared with the reference fingerprints of Semen Cassiae and fried Semen Cassiae,the similarities were 0.94 and 0.67 respectively,and when the 12 batches of fried Semen Cassiae were compared with the reference fingerprints of Semen Cassiae and fried Semen Cassiae,the similarities were 0.92 and 0.62 respectively.Both had significant differences(P＜0.01).Compared with Semen Cassiae,the contents of 6 components including aurantio-obtusin in fried Semen Cassiae increased,of which emodin,chrysophanol and physcion had statistical significance(P＜0.01 or 0.05).Conclusion:Emodin,chrysophanol and physcion without hydrolysis can be used to evaluate the content changes of internal components in Semen Cassiae before and after frying.The chemical fingerprints can be used for identification.