CD C25B基因表达对膀胱癌患者临床预后的影响

摘要

Objective To investigate the difference of the pathological features of CDC 25B ex‐pression in bladder cancer (BCa) tissues by gene expression compilation database ,and to determine the effect of CDC25B expression on the specific survival rate and overall survival rate of patients with BCa· CDC25B affects the possible pathways of BCa development· Methods We downloaded NCBI＇s BCa public data set ,and carried out a retrospective and prognostic analysis· T hen we used GSEA to analyse the relevant pathways affected by CDC25B· Results T he expression level of CDC25B was associated with disease progression (P＜0·000 1) ,grade (P＜0·000 1) ,and T stage (P＜0·000 1) in patients with BCa· Compared with the low expression group of CDC25B ,patients with high ex‐pression of CDC25B had higher grades· And T stage was more advanced ,disease progresses faster· T he overall survival (P＜0·000 1 , H R＝0·333 3 ,95% confidence interval :0·204 2‐0·544 0 ) and specific survival (P＜0·000 1 , H R＝0·170 5 ,95% confidence interval :0·084 6‐0·343 7 ) of BCa patients with CDC25B overexpression were both significantly lower than those with CDC 25B low ex‐pression· GSEA analysis showed that CDC25B high expression samples were enriched with sperm formation ,mitotic spindle formation ,U V response ,E2F signal pathway ,G2 M Point ,KRAS relat‐ed genes· Conclusions CDC25B is closely related to the clinicopathological features of BCa ,and can be used as a marker to judge the extent and prognosis of BCa invasion and metastasis·%目的 通过公共基因表达数据库,探讨细胞周期蛋白25B(CDC25B)的表达与膀胱癌(BCa)患者临床病理特征的关系;确定CDC25B高表达及低表达对BCa患者术后肿瘤特异性生存期与总生存期的影响;预测CDC25B影响BCa发生、发展的可能通路. 方法 下载美国国立生物技术信息中心的BCa公共数据集,分析BCa基因表达谱资料及其对应的临床信息,卡方检验用于探究CDC25B的高、低表达与BCa患者临床病理参数的相关性,Log‐rank检验用于比较BCa患者的总生存期和肿瘤特异性生存期.基因集富集分析(GSEA )则用于探究BCa受CDC25B影响的相关途径及信号通路. 结果 CDC25B表达水平与BCa患者的疾病进展(P＜0.000 1) 、分级(P＜0 .000 1 ) 、T分期(P＜0 .000 1)相关,相较于CDC25B低表达组,CDC25B高表达患者的分级高,T 分期更趋向于晚期,疾病进展更快;预后分析结果显示CDC25B高表达组患者的总生存期( H R＝0·333 3 ,95%CI :0·204 2～0·544 0 ,P＜0·001)和肿瘤特异性生存期(HR＝0·170 5 ,95% CI :0·084 6～0·343 7 ,P＜0·001)明显差于低表达组;GSEA分析得到CDC25B高表达样本富集了与精子形成、有丝分裂纺锤体形成、紫外线响应、G2 M检查点、E2F信号通路、KRAS信号通路、肌形成通路相关的基因组. 结论CDC25B与BCa临床病理特征有密切相关性,有望作为判断BCa侵袭转移程度及预后的指标.