不同冷冻温度、时间对冻干血小板回收率和残留水的影响

摘要

Objective To investigate the effect of varied freezing temperature and time on the recovery and residual water in the freeze-dried platelets. Method The freeze-drying processes of temperature and time were divided into three types (A, B and C). The platelets were freeze-dried and rehydrated using the three types of freeze-drying processes, respectively. The residual water content of the lyophilized platelets was detected with dry-weighing method.The differences of the recovery rate and residual water,and the MPV and PDW values in the freeze-dried platelets were compared. Result The difference between freezing temperature/time for the freeze-drying platelets,the difference of the MPV values after the rehydration of freeze-dried platelets were not statistically significant (P>0.05). The PDW value of freeze-dried platelets after rehydration between type A and type C showed no significant difference (P>0.05), while that of type B was high (P<0.05). The recovery rate of types A, B and C rehydration platelets was (81.72±12.43)%,(81.40±8.79)%and (94.37±7.02)%, respectively, with no significant difference among the three types (P>0.05). The lyophilized platelets residual water contents in types A, B and C were (6.55±0.96)%,(7.10±1.20)%,and (5.18±0.89)%, respectively,with no significant difference among the three types (P>0.05). Conclusion Based on the residual water and recovery of the freeze-dried platelets by using various processes,freeze-drying temperature and time should be optimized.%目的：探讨不同冷冻温度、时间对冻干血小板回收率及残留水的影响。方法依据温度、时间不同的冻干流程分3组（A组、B组和C组），分别进行血小板冻干、复水试验。用干燥称重的方法检测冻干血小板残留水含量，比较不同冷冻温度、时间对冻干血小板回收率和残留水的差异，同时比较不同冷冻温度、时间冻干血小板复水后MPV、PDW的差异。结果不同冷冻温度、时间条件下，冻干前与冻干复水后血小板MPV结果的差异均无统计学意义（P>0.05）；冻干前与复水后血小板PDW结果比较，A组和C组的差异均无统计学意义（P>0.05），而B组冻干复水后血小板PDW高于冻干前血小板PDW，且差异有统计学意义（P<0.05）；A组、B组和C组冻干血小板复水回收率分别为（81.72±12.43）%、（81.40±8.79）%和（94.37±7.02）%，3组冻干血小板复水回收率的差异均无统计学意义（P>0.05）；A组、B组和C组冻干血小板残留水分别为（6.55±0.96）%、（7.10±1.20）%和（5.18±0.89）%，3组冻干血小板残留水含量的差异均无统计学意义（P>0.05）。结论3组不同冻干温度、时间冻干血小板残留水、复水回收率相比，选择C组血小板冻干流程为后续研究血小板冻干程序，为血小板冻干流程的建立奠定基础。