汕头献血人群HBV DNA定量检测结果与乙肝两对半模式分析

摘要

Objective To analyze the HBV-DNA quantitative test results and serum HBV markers in blood donors. Methods The positive HBsAg was detected as HBV markers in blood donors and HBV-DNA was tested by fluorescent quantitative-PCR, and samples over1000 IU/ml was subjected to DNA sequencing.Results9 HBV-M modes in the measured samples were noted and each of the modes presented the active DNA replication in a certain degree.Conclusion There are a higher viral replication activity in HBV carriers in blood donors, to which more attention should be paid. The genotype B of HBV might be the endemic character in the local blood donors.%目的：分析献血人群中HBV DNA定量检测结果与乙肝两对半模式的关联。方法对于献血者血液筛查出来的HBsAg阳性者进行乙肝两对半检测、HBV DNA荧光定量PCR检测，对于荧光定量PCR高于1000 IU/ml的进行DNA测序。结果所测样本存在9种HBV-M模式，各种模式都有一定比率的DNA活动性复制，测序标本均为HBV B型。结论献血人群的乙肝病毒携带者中存在较高的病毒活动性复制，应予以重视和进一步研究，HBV基因型全部为B型，可能是本地献血人群的分布特点。