邢台地区血小板志愿捐献者HPA基因资料库的建立

摘要

Objective The HPA genotyping ofplatelet donors in xingtai area was performed,and the HPA genotype of the platelet donors was established.Method PCR-SSP method was used to randomly select 150 platelet donors who carry out a total of 14 antigens of HPA-1-6 and HPA-15.Result The gene frequencies were found to be 0.953 and 0.010 for HPA-1aand-1b:0.951 and 0.086 for HPA-2a and-2b:0.596 and 0.394 for HPA-3a and-3b:1.000 and 0.000 for HPA-4a and-4b:0.967 and 0.015 for HPA-5a and-5b:0.986 and 0.010 for HPA-6a and-6b:0.475 and 0.506 for HPA-15a and-15b.Conclusion The gene frequencies of HPA-1-6 and HPA-15 antigens in xingtai area of Hebeiprovince coincide with the Hardy-Weinberg genetic law,which shows difference among nationalities and regions.This is the first establishment of HPA genotype database in xingtai.%目的 对邢台地区血小板志愿捐献者进行人类血小板抗原(human platelet antigen,HPA)基因分型,建立已知HPA基因型的血小板捐献者资料库.方法 采用聚合酶链反应-序列特异性引物(polymerase chain reaction-sequence specific primers,PCR-SSP)方法对随机抽取的1 50例血小板捐献者进行HPA-1～6和HPA-15抗原系统共14个抗原基因分型.结果 邢台地区血小板志愿捐献者HPA的基因频率分别为:HPA-1a 0.953,1b 0.010;2a 0.953,2b 0.086;3a0.596,3b 0.394;4a 1.000,4b 0;5a 0.967,5b 0.015;6a0.986,6b 0.010;15a 0.475,15b 0.506.结论 邢台地区血小板志愿捐献者HPA-1～6和HPA-15抗原系统的基因频率符合Hardy-weinberg遗传定律,与其他地区和国家的同类资料相比显示出种族和地域性差异,应建立邢台地区已知HPA基因型的血小板捐献者资料库.