[目的]研究CD4+ CD25+调节性T细胞(CD4+ CD25+Treg细胞)和Foxp3基因表达与子痫前期发病的相关性.[方法]选择住院确诊的妊娠期高血压孕妇12例(A组)、轻度子痫前期(MPE)孕妇14例(B组)、重度子痫前期(SPE)孕妇15例(C组),30例正常妊娠晚期孕妇为对照组(D组),应用流式细胞术检测并比较四组外周血中CD4+ CD25+ Treg和 Foxp3的表达水平.[结果]A组、B组和C组孕妇外周血CD4+ CD25+ Treg 和Foxp3表达水平均显著低于对照组(P<0.05);C组外周血CD4+ CD25+ Treg和Foxp3表达水平显著低于A组和B组(P<0.05).[结论]子痫前期患者体内存在CD4+ CD25+ Treg细胞的异常,CD4+ CD25+Treg细胞的数量的减少和Foxp3表达的降低使CD4+ CD25+Treg细胞免疫抑制功能减弱,从而使母胎免疫耐受失衡,导致子痫前期的发生.%[Objective] To investigate the relationship between CD4+ CD25 + Treg cells, Foxp3 gene ex pression and the pathogenesis of preeclampsia. [Methods] Twelve pregnant women with gestational hyperten sion(group A) , 14 mild preeclampsia(MPE) pregnant women(group B) , 15 severe preeclampsia(SPE) preg nant women(group C) and 30 normal pregnant women(group D) were chosen in hospital. Flow cytometry was used to detect and compare CD4 + CD25 + Treg cells and expression level of Foxp3 in peripheral blood among 4 groups. [Results] The CD4 + CD25 + Treg cells and Foxp3 expression level in peripheral blood in group A, B and C were significantly lower than those in control group( P ＜0.05). The CD4 + CD25 + Treg cells and Foxp3 expression level in peripheral blood in group C were significantly lower than those in group ∧ and B( P ＜0.05). [Conclusion] Preeclampsia patients have abnormal CD4 + CD25 + Treg cells. The decrease of CD4 + CD25 +Treg cells and Foxp3 expression attenuates the immune suppression function of CD4 + CD25 + Treg cells, thus maternal-fetal immune tolerance becomes imbalance which results in the occurrence of preeclampsia.
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