黄体酮对创伤性脑损伤大鼠神经细胞凋亡的影响

摘要

To investigate the effect of progesterone administration on cell apoptosis following traumatic brain injury ( TBI) in rats and to explore if' it protect brain tissues. Methods Male wistar rats were randomlyallocated into f'our groups (24 rats each group) including sham,traumatic brain injury ( TBI) , progesterone treated ( P-TBI) , and dimethyl sulphoxide ( DMSO)treated (D-TBI) group. Every group was divided into four subgroups at 1 d, 3 d, 5 d and 7 d postinjury (6 rats each subgroup). Parietal brain contusion was adopted using Freeney's weightdropping method. Apoptosis was detected by in situ immunohistochemical staining ( TUNEL) at each coresponding time point. Results The result showed the number of apoptotic cells increased since 1 d after TBI, peaking at 7 d postinjury. The number of neurocyte apoptosis can be decreased by injections of progesterone ( P ＜ 0. 05 ) . Conclusions In the early phase of brain contusion, the number of neurocyte apoptosis were up-regulated obviously. Progesterone administration can inhibits the neurocyte apoptosis following TBI in tats and protect brain tissues effectively.%目的 观察黄体酮对脑外伤后神经细胞凋亡的影响,探讨其对脑外伤(TBI)继发性脑损伤是否存在保护作用.方法 雄性Wistar 大鼠随机分为无脑损伤的假手术(sham)组、脑损伤(TBI)组、脑损伤后注射黄体酮治疗(P-TBI)组及注射二甲基亚砜(DMSO)溶剂(D-TBI)组,每大组24 只,再分1 d、3 d、5 d 和7 d 四个小组,每小组6 只.采用Freeney 法造成鼠脑挫裂伤模型,在伤后四个不同时相点用TUNEL 染色法分别检测四组大鼠脑组织中神经细胞凋亡情况.结果 创伤性脑损伤后凋亡细胞数量在TBI 第1 d 明显增加,TBI后第7 d 神经细胞凋亡达到高峰.伤后注射黄体酮治疗组神经细胞凋亡指数明显下降(P ＜0.05).结论 大鼠TBI 后周围脑组织神经细胞凋亡在伤后持续增加,注射黄体酮能抑制细胞凋亡,对脑组织有一定保护作用.