Objective To explore the changes of interleukin-32 and interleukin-6 in serum of patients with hepatitis B virus infection and its clinical significance. Methods The serum IL-32 and IL-6 of 92 HBV-infected patients and 30 healthy individuals were detected by ELISA. Results (l)There was statistical difference in serum IL-32 between the patient in hepatitis B group and control group (F=108.494, P＜0.001). The level of serum IL-32 in patient with AHB is the highest among the subjects. The level of IL-32 in serum was gradually increased in patients with mild, moderate and severe chronic hepatitis B. The level of serum IL-6 in hepatitis B group has significant difference compare with control group (F=139.256, P＜0.001), moreover the level of IL-6 in serum gradually increased in patients with mild, moderate and severe chronic hepatitis B. (2)The serum levels of IL-32, IL-6 in the HBV-infected patients with HBV DNA positive group were higher than those observed in the control group, however, no significant difference was observed between the groups. The differences among various HBV viral load groups were also not statistically significant (P＞0.05). (3)The serum level of IL-32 was positively correlated with IL-6, the correlation coefficient r was 0.70(P＜0.05). Conclusion (1)The results indicate that the level of IL-32, IL-6 increases in HBV-infected patients. The level of IL-32, IL-6 increases with the aggravation of inflammation of liver, indicating that IL-32, IL-6 play an important role in inflammatory response and are responsible for liver inflammatory injury process and diseases progression in HBV-infected patients. (2)It seems that the changes in serum IL-32 and IL-6 were not related to the HBV replication status.%目的 探讨乙型肝炎患者血清白细胞介素(IL)-32IL-6的变化及其临床意义.方法 对92例乙型肝炎患者和30例健康者用ELlSA法检测外周血清IL-32和IL-6水平变化.结果 (1)乙型肝炎组与对照组血清IL-32水平比较差异有统计学意义(F=108.494,P<0.001),急性乙型肝炎(AHB)组血清IL-32水平最高,慢性乙型肝炎(CHB)组随轻、中、重分型逐渐升高;乙型肝炎组与对照组血清IL-6水平比较差异有统计学意义(F=139.256,P<0.001),依AHB、CHB轻、中、重度逐渐升高.(2)HBVDNA阳性组IL-32、IL-6水平较阴性组为高,差异无统计学意义;高、中、低病毒载量组之间血清IL-32、IL-6水平比较差异无统计学意义(P>0.05).(3)血清IL-32水平与IL-6水平呈正相关(r=0.70,P<0.05).结论 (1)乙型肝炎患者外周血IL-32、IL-6水平升高,且随炎症程度加重呈上升趋势,推测IL-32、IL-6可能在炎症反应中发挥重要作用,IL-32、IL-6参与了乙型肝炎患者肝组织损伤及病情发展的过程.(2)血清IL-32、IL-6水平变化与HBV复制水平无关.
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