Objective To investigate the therapeutic effects of ω -3 polyunsaturated fatty acids ( PUFAs) during the early stage of acute pancreatitis. Methods AR42J pancreatic acinar cells were divided into three groups: A, untreated control; B, pretreated with the ω -3 PUFA docosahexaenoic acid ( DHA) and stimulated with cerulein to simulate the inflammatory response of acute pancreatitis; and C, stimulated with cerulein only. Expression of inflammatory cytokines (TNFa and IL -6) and a regulator of reactive oxygen species ( UCP2) was measured by real - time PCR. Expression of the NF - ΚB p65 subunit in nuclear protein was detected by Western blotting. Necrotic and ap-optotic cells were detected by staining with Hoechest33342 and propidium iodide, respectively. Results Cerulein -stimulated cells had significantly higher levels of TNFa, IL -6, and UCP -2 mRNA expression, NF - ΚB p65 protein, and cell necrosis (groups B and C vs group A, P<0. 05 ). DHA pretreatment significantly reduced the levels of all cerulein - stimulated changes ( group B vs group C, P < 0. 05 ). Conclusion ω -3 PUKAs may inhibit the inflammatory reaction during the early stage of acute pancreatitis.%目的 探讨ω-3多不饱和脂肪酸(ω-3 PUFAs)对急性胰腺炎早期炎症反应的作用.方法 培养胰腺腺泡细胞系AR42J,随机分为A组(空白对照)、B组(ω-3 PUFAs预处理后蛙皮素刺激)、C组(仅用蛙皮素刺激);实时荧光定量PCR检测各组细胞肿瘤坏死因子(TNF)α、白细胞介素(IL) -6和线粒体解偶联蛋白-2(UCP-2)mRNA表达;Western Blot检测细胞核中NF-κB p65亚单位的含量;Hoeehst33342/PI染色检测细胞坏死.结果 B组TNFα、IL -6、UCP-2 mRNA表达水平、细胞核NF - κBp 65含量及细胞坏死率与C组相比均明显减少,差异有统计学意义[(0.69±0.10)vs(1.34±0.19),P＜0.001;(0.69±0.06) vs( 1.39±0.06),P＜0.001;(0.58±0.12)vs(1.26±0.07),P＜0.001;(0.54±0.09) vs (0.92±0.09),P＜0.001;(0.20±0.01)vs(0.35±0.03),P＜0.001;F =62.718～157.426,P均＜0.05].而B组、C组TNFα、IL -6、UCP-2 mRNA表达水平、细胞核NF-κBp65亚单位含量及细胞坏死率均高于A组,差异有统计学意义[(0.69±0.10)、(1.34±0.19) vs (0.34±0.05),P=0.003、P＜0.001;(0.69±0.06)、(1.39±0.06) vs (0.23±0.03),P=0.001、P＜0.001; (0.58±0.12)、(1.26±0.07)vs(0.32±0.08),P=0.003、P＜0.001; (0.54 +0.09)、(0.92±0.09)vs(0.26±0.12),P＜0.001、P＜0.001; (0.20±0.01)、(0.35±0.03) vs (0.13±0.03),P=0.024、P＜0.001;F=62.718～157.426,P均＜0.05].结论 ω-3 PUFAs可能抑制急性胰腺炎早期炎症反应.
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