大鼠股骨骨折合并脑外伤时HIF -1α及 Cbfα1的血清表达及意义

摘要

Objective To investigate the expression and significance of hypoxia inducible factor 1 alpha(HIF - 1α)and core binding factor alpha 1(Cbfα1)in rats with femoral fracture and traumatic brain injury in rats. Methods Seventy SD rats(three months old)were divid-ed into sham operation group(group A),femoral fracture group(group B),traumatic brain injury group(group C)and fracture with brain injury group(group D). Twenty - four hours after model establishment,brain tissue of group C and D were sampled and HE stained to determine the de-gree of brain trauma. Serum levels of HIF - 1 α and Cbf α 1 were detected by ELISA on day 1,day 3 and at week 1,2,3,4,and 5 after model estalishment. Results There were significant differences in the expressions of HIF - 1 α and Cbf α 1 in different time points in group B,C and D groups( P < 0. 05). At the same time point,the expressions of HIF - 1 α and Cbf α 1 were higher in group B,C and D than those in group A( P < 0. 01);and the expressions were higher in group D than those in group B and C(all P < 0. 05). Conclusion Serum levels of HIF -1 α and Cbf α 1 are enhanced in rats after fracture and brain injury,and the expressions are more prominent in rats with fracture combined with traumatic brain injury;which may be one of the important factors of accelerated fracture healing after traumatic brain injury.%目的：探讨大鼠股骨骨折合并脑外伤时低氧诱导因子-1α（HIF -1α）及核心结合因子α1（Cbfα1）在血清中的表达及意义。方法选用70只3月龄健康 SD 大鼠，随机分为假手术组（A 组），股骨骨折组（ B 组），脑外伤组（C 组）和骨折合并脑外伤组（D 组），分别进行造模。造模术后24 h 取 C、D 组脑组织行苏木素-伊红染色判断脑外伤程度。1 d、3 d、1周、2周、3周、4周、5周通过酶联免疫吸附法（ELISA）检测各组 HIF -1α及 Cbfα1的血清表达。结果B、C、D 组内不同时间段 HIF -1α及 Cbfα1表达比较，差异有显著性（ P <0.05）；同一时间段 B、C、D 组表达均明显高于 A 组，D 组高于 B、C 两组，差异均有显著性（ P <0.05）。结论 HIF -1α及 Cbfα1在大鼠骨折和脑外伤后均有较明显的表达，在骨折合并脑外伤后表达更加突出，这可能是造成脑外伤后加速骨折愈合的重要因素之一。