急性早幼粒细胞白血病患者白血病细胞免疫表型分析

摘要

Objective Our retrospective study was aimed to discuss the biological significance of the immune phenotype purified and sep-arated from patients with acute promyelocytic leukemia cells. Methods Clinical data of patients was retrospectively analyzed. 71 cases of acute promyelocytic leukemia patients first diagnosed were divided according to the test results on leukemia cells triggered immune phenotype by experi-enced medical examiner. Then the three established diagnostic criteria for classification of acute leukemia( FAB)were analyzed. The value of phe-notype CD64,CD117,CD9 and CD56 was analyzed and discussed by group. Results A total of patients were retrospective analyzed. In acute promyelocytic leukemia cells in leukemia cells,myeloperoxidase( myeloperoxidase,MPO),CD33 and CD13 expression rate was the highest,reac-hing 100%,followed by CD123,CD9,CD45,CD117 ,CD19,CD64 and CD56. MPO,CD33 and CD13 were expressed in all patients in both APL,their expression level was respectively 84. 7%,92. 4% and 83. 5%,with higher strength. and the uniform expression of MPO was distribu-ted,the uniform expression of CD13,CD33,CD34,CD117,CD64 and CD56 and FAB classification was independent( P >0. 05);the expression of CD9 and FAB classification are interrelated( P 0.05）。CD9的阳性表达与FAB分型之间是相互关联的（ P <0.05）。结论在所有的APL患者中MPO、CD13和CD33均有阳性表达，MPO在白血病细胞中阳性表达分布较为均匀，CD13在白血病细胞中的阳性表达分布较为集中。而大部分的免疫表型与FAB分型中的M3a和M3b之间无明显的线性关系。