肾移植与肝移植术后抗 HLA 抗体产生的比较研究

摘要

目的：研究肾移植和肝移植术后患者抗 HLA 抗体产生几率。方法研究对象为首都医科大学附属北京友谊医院2007年10月至2009年12月的肾移植患者371例和2012年1月至2014年3月肝移植术后患者82例。采用美国莱姆德公司和 GTI 公司提供的酶联免疫吸附试验（ELISA）法检测肾移植患者群体反应性抗体。结果371例肾移植患者中，29例患者 PRA 阳性，占7.82％，342例患者 PRA 阴性，占92.18％。29例 PRA 阳性患者中7例抗 HLA - I 例抗体阳性患者，5例抗 HLA - II 例抗体阳性患者，17例抗 HLA - I ＋ II 例抗体阳性患者。82例肝移植患者中，7例患者PRA 阳性，占8.54％；75例患者 PRA 阴性，占91.46％。7例 PRA 阳性患者中1例抗 HLA - I 例抗体阳性患者，4例抗HLA - II 例抗体阳性患者，2例例抗 HLA - I ＋ II 例抗体阳性患者。肾移植术后与肝移植术后抗体产生率无统计学意义（χ2=0.576，P ﹥0.05）。结论肾移植与肝移植术后抗 HLA 抗体产生几率无显著差异。%Objective To investigate the generation probability of panel reaction antibody(PRA)following kidney and liver transplanta-tion. Methods 371 kidney transplantation and 82 liver transplantation patients were available for PRA detection with ELISA kit.(GTI,USA). The kidney and liver transplantations were performed during Oct 2007 to Dec 2009 and Jan 2012 to Mar 2014 respectively. Results In the 371 kidney transplantation patients,29 showed PRA positive(7. 82% ,29 / 371),including 7 anti - HLA class I antibody,5 anti - HLA class II anti-body and 17 anti - HLA class I + II antibodies. In the 82 liver transplantation patients,7 showed PRA positive,(8. 54% ,7 / 82),including 1 an-ti - HLA class I antibody,4 anti - HLA class II antibody,and 2 anti - HLA class I + II antibodies. The incidence of PRA production between kid-ney and liver transplantation patients had no statistically significant difference(χ2 = 0. 576,P ﹥ 0. 05). Conclusion No statistically significant difference of PRA following kidney and liver transplantation.