IL-1β-511基因多态性与妇科手术患者电刺激痛觉敏感性的关系

摘要

探讨IL-1β-511基因多态性与妇科手术患者术前痛阈及耐痛阈的关系.方法择期腹式子宫全切或子宫肌瘤剔除术患者250例,年龄20～50岁,ASA Ⅰ或Ⅱ级.术前应用电刺激仪进行痛阈及耐痛阈测定,采用聚合酶链反应-限制性片断长度多态性技术(PCR-RFLP)进行IL-1β-511基因多态性位点检测,根据基因型将患者分为三组:C/C组(野生纯合子组),C/T组(杂合子组),T/T组(突变型纯合子组).结果共208例患者检测出基因型,其中C/C组54例,C/T组107例,T/T组47例,三组痛阈和耐痛阈组间差异无统计学意义.结论IL-1β-511基因多态性不影响妇科患者电刺激痛觉敏感性.%Objective To evaluate the relationship between IL-1β-511 gene polymorphism and preoperative pain threshold, pain tolerance threshold of women. Methods A total of 250 women patients, 20-50 yr, ASA I or Ⅱ, scheduled for elective myomectomy or total hysterectomy were studied. Preoperatively, the pain threshold and pain tolerance threshold were measured using the electrical stimulus. The polymorphic sites of the IL-1β-511 allele were analyzed by polymerase chain reaction-restriction fragment length polymorphism. The patients were assigned into 3 groups according to their genotypes:groupC/C(wild homozygote);group C/T(mutation hetorozygote) and group T/T (mumfion homozygote). Results Among the 208 subjects,54 women were wild homozygous (C/C), 107 were heterozygous (C/T), and 47 were mutant homozygous (T/T). No difference in IL-1β-511 gene polymorphism was found among the three sub-group in the pain threshold and pain tolerance threshold. Conclusion The frequency of I1-1β-511T allele is 48% in Chinese gynaecologic patients. There were no correlation was found between I1-1B-511 polymorphism and pain threshold and pain tolerance threshold in Chinese women.