维生素C对糖尿病大鼠肾缺血-再灌注损伤的影响

摘要

Objective To investigate the protective effects of ascorbic acid on renal ischemia/reperfusion in STZ-induced diabetic rat. Methods Thirty-two SD rats were randomly divided into four groups with 8 rats each. The rats in group A, B, and C were induced diabetes by STZ,groupB and group C were exposed to ischemia-reperfusion. The rats in group A and group B were given normal saline 0. 5 ml/d i.P. for 15 days, group C were given ascorbic acid 200 rng· kg-1·d-1 i.P. for 15 days, group D was control. Results Compared with those in group B, the concentration of serum BUN,SCr and MDA were significant higher in group C(P＜0. 05). The SOD level in group B was obvious lower than that in group C(P＜0.05). In group C the tubular cell damage was lesser, the expressions of Cytc and caspase-3 were lower in group C. Conclusion Vc treatment can reduce renal injury and improve renal function by reducing lipid oxidation and cell apoptosis caused by IR in STZ-induced diabetic rats.%目的 观察维生素C对链脲佐菌素(STZ)诱导糖尿病大鼠肾缺血-再灌注损伤的影响.方法 32只SD大鼠随机均分为四组:A、B、C组为糖尿病大鼠,B、C组行缺血-再灌注处理,A、B组每天腹腔注射生理盐水0.5 ml,C组注射维生素C 200 mg/kg(0.5 ml),D组为空白对照组.观察血糖、尿素氮(BUN)、肌酐(Cr)、丙二醛(MDA)、超氧化物歧化酶(SOD)、细胞色素C(Cytc)、半胱天冬酶-3(caspase-3)的变化.结果 B组BUN、Cr、MDA含量较C组明显升高(P＜0.05),S0D的活性明显降低(P＜0.05).C组caspase-3和Cytc的表达明显减少,肾小管损伤、坏死显著减轻.结论 维生素C能减轻STZ诱导糖尿病大鼠肾缺血-再灌注损伤的氧化应激,抑制细胞的凋亡及改善肾功能.