七氟醚预处理对肝脏缺血-再灌注损伤及PI3K/Akt信号通路的影响

摘要

目的 探讨七氟醚预处理对肝脏缺血-再灌注损伤及磷脂酰肌醇3激酶/蛋白激酶B(PI3K/Akt)信号通路的影响.方法 48只成年SD大鼠随机分为假手术组(SH组)、缺血-再灌注组(IR组)、七氟醚预处理组(SI组)和七氟醚Wortmannin组(SW组),每组12只.除SH组外,IR组、SI组和SW组建立70％肝脏缺血-再灌注模型.检测大鼠血清谷草转氨酶(AST)和谷丙转氨酶( ALT)含量,并取再灌注的肝组织用ELISA法测IL-1β浓度,用Western Blot法测定肝组织的p-PI3K、p-Akt(ser473)、Akt等表达量;用HE染色进行病理学检查,观察肝细胞损伤及坏死情况.结果 与SW组比较,SI组ALT、AST和IL-1β浓度明显降低(P＜0.05),且p-PI3K、p-Akt(ser473)表达量明显增加(P＜0.05),肝组织损伤减轻.结论 七氟醚对肝脏缺血-再灌注损伤有保护作用,可能通过激活PI3K/Akt信号通路起作用.%Objective To investigate the effects of sevoflurane preconditioning on hepatic ischemic reperfusion injury (HIRI) and PI3K/Akt signaling pathway. Methods Forty-eight adult male SD rals were equally randomized imo 4 groups; sham group (SH group). ischemic reperfusion injury group ( 1R group), sevoflurane preconditioning + HIRI group ( SI group), sevoflurane preconditioning+HIRI+wortmannin group (SW group). All rals suffered from segmental hepatic ischemic (70%) except the rats in the SH group served as cootroll. The serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), the liver interleukin-lβ (1L-1β) concentration, and the expressions of phosphory'-iled FI3K, Akt, and Akl were mersured. The injury of hepatic cells was delected Results The levels of ALT, AST, and IL-1β increased in the IR, SI, and SW groups than in the SH group (P<0. O5). The levels of ALT, AST. and IL-1β increased in the IR and SW groups than in the SI group (P<0. 05). The expression of phosphorylaied PI3K and Akt increased in the IR. SI, and SW groups than in the SH group (P<0. 05). The expression of phosphorylaied PI3K and Akt decreased in the IR arid SW groups lhan in the SI group (P<0. 05). Conclusion Sevoflurane preconditioning reduces hepatic- ischetnie reperfusion injury in rats, which may, be .attributed to the activation of the P13K/Akt signal pathway