Objective:With the model rats of acute SCI, the paper investigates that the electro - acupuncture affects the expression of neural cell apoptosis and changes related genes for PARP - 1 after spinal cord injury of rats. The paper tries to show that the mechanisms of electro - acupuncture on spinal cord injure and the study of related important factors of mitochondrion excitement apoptosis way. Methods: Acute spinal cord injury models by Allen's preparation, the rats were divided into acupuncture group and drug group (Caspase -3 inhibitor group), model group, sham operation group. Apoptotic cells were marked by Tunel method. Results:Tunel labeling results showed that spinal cord injury after 6h, PARP - 1 expression in the model group appeared, 1 d expression was the highest,on the 3 d,7 d it gradually decreased, on the 14 d it was the lowest. Model group expression levels were higher than that in sham operation group and drug group on the 1 d, 3 d ( P ＜ 0. 05 ). Expression of electro - acupuncture group was lower than that in model group on the 3 d (P ＜ 0. 05 ). Conclusion:First: Apoptosis occurs in rats after acute spinal cord injury. Spinal cord injury is the main pathological mechanism. Second: The expression of PARP - 1 in rat neurons increased gradually after spinal cord injury, suggesting that PARP - 1 may be involved in spinal cord injury. Third: Electro - acupuncture inhibits apoptosis of neural cells and reduces spinal cord injury by inhibiting neurons expression of PARP - 1 in spinal cord injury.%目的:采用大鼠急性脊髓损伤模型,观察电针对脊髓损伤后神经细胞凋亡与其相关基因PAfuP1全长表达影响及变化规律,试图从线粒体启动凋亡途径角度揭示电针治疗脊髓损伤作用机制.方法:急性脊髓损伤模型以Allen's法制备,将大鼠分为电针组、药物组(Caspase-3抑制剂组)、模型组、假手术组.对凋亡细胞采用Tunel法予以标记.结果:Tunel标记结果表明:脊髓损伤后6 h,PAfuP-1全长在模型组开始有表达,1 d表达量最高,在3 d、7 d逐渐下降,14 d最低.模型组在1 d、3 d表达量分别高于假手术组和药物组(P<0.05).电针组在3 d表达量低于模型组(P<0.05).结论:①细胞凋亡现象发生在大鼠急性脊髓损伤后,这是脊髓继发性损伤主要病理机制;②PARP-1全长在大鼠脊髓损伤后神经元中表达量随着时间推移逐渐增加,提示PAfuP-1全长可能参与了脊髓继发性损伤;③电针通过抑制PARP-1大鼠脊髓损伤神经元中PARP-1全长的表达,从而抑制了神经细胞的凋亡,减轻脊髓继发性损伤.
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