通督调神与疏肝解郁法对抑郁模型大鼠行为学及 HPA轴的影响及其差异

摘要

目的：观察通督调神、疏肝解郁两种针刺方法对抑郁模型大鼠行为学及下丘脑－垂体－肾上腺轴（ HPA轴）的影响，探讨二者抗抑郁作用及其机制差异。方法：SD雄性大鼠随机分为正常组和模型组。模型组采用慢性不可预见性温和性应激（ CUMS）结合孤养方法造模，2周后筛选出模型制备成功的大鼠，再随机分为模型组、西药组、通督调神组、疏肝解郁组。各治疗组接受有关治疗，6日／周，持续3周。每隔7日进行1次行为学检测，3周后用放免法检测HPA轴相关指标。结果：①造模14日及治疗3周末，与正常组比较，模型组大鼠体重、旷场实验得分、糖水消耗百分比均明显降低（P＜0．05），表明模型成功而稳定。②治疗3周末，两针刺组体重、旷场实验得分、糖水消耗百分比较模型组均明显增加（P＜0．05）。除通督调神组旷场实验水平得分高于疏肝解郁组（P＜0．05）外，两针刺组各项行为学指标间无统计学差异。③治疗3周末，模型组大鼠下丘脑促肾上腺皮质激素释放激素（ CRH ）、垂体促肾上腺皮质激素（ACTH）及肾上腺皮质酮（CORT）含量较正常组均明显增加（P＜0．05），两针刺组较模型组均明显降低（P＜0．05），且其间无统计学差异。结论：通督调神与疏肝解郁法均能明显改善抑郁大鼠的行为学指标，其中通督调神法略优；二者均能够调节HPA轴的功能亢进且作用相当，这可能是其抗抑郁的机制之一。%Objective:To observe the influences of Tongdu Tiaoshen method and Shugan Jieyu method on the changes of behavior and hypothalamus -pituitary-adrenal axis in CUMS depressive model rats ,and to investi-gate the differences of two acupuncture methods on the antidepressant function and therapeutic effect .Methods:SD male rats were randomly divided into a normal group and a model group .Depressive model rats were estab-lished by using chronic unpredictable mild stress stimulation combined with lonely breeding .After two weeks , the successful depressive model rats were chosen and randomly divided into a model group ,a western medicine group ,a Tongdu Tiaoshen group and a Shugan Jieyu group .Three treatment groups received relevant treatments for 3 weeks,6 times per week.The rat’s behavior was observed once a week and related hormones of hypothala-mus-pituitary-adrenal axis were detected by radioimmunoassay techniques after 3 weeks.Results:Compared with the normal group ,the body weight ,consumption of sucrose and scores of open -field test of the model group declined significantly on the 14th day after making models and after 3 weeks of the treatment(P<0.05).It was shown that the models were duplicated successfully and stably .Compared with the model group , the body weight,consumption of sucrose and scores of open -field test of two acupuncture groups were significantly in-creased after 3 weeks of the treatment(P<0.05).The result also showed that Tongdu Tiaoshen group was sig-nificantly higher than Shugan Jieyu group on the level movement scores of the open -field test(P<0.05), but other behavioural indicators showed no statistical differences .On the three indexes of CRH hypothalamus , pitui-tary ACTH and adrenal CORT contents , the model group showed no significant increase compared with the nor-mal group after 3 weeks of the treatment ( P<0 .05 ) , but the two acupuncture groups were significantly lower than the model group and there was no statistical difference between two acupuncture groups ( P<0 .05 ) .Con-clusion:The two acupuncture methods can obviously improve the behavioural indicators of depressive model rats.Moreover, Tongdu Tiaoshen method is slightly better than the method of Shugan Jieyu .The two acupunc-ture methods can adjust the hyperactivity of HPA axis , which may be one of the antidepressant mechanisms .