全反式维甲酸对大鼠缺血再灌注肾损伤的保护作用

摘要

目的 研究全反式维甲酸(ATe)对大鼠缺血再灌注肾损伤的保护作用.方法 24只SD大鼠分为4组(1)正常对照组;(2)假手术组;(3)模型组:建立肾缺血再灌注模型,不做处理.(4)维甲酸处理组:造模前用ATRA灌胃,连续7d.造模后24 h处死大鼠,留取血清及肾组织标本.检测血尿肌酐、血清肿瘤坏死因子-o(TNF-α)和干扰素-γ(IFN-γ)水平,肾组织切片染色,肾小管评分判断肾损害程度,检测肾组织凋亡情况.结果 与模型组相比,维甲酸处理组的大鼠血肌酐下降,尿肌酐、肌酐清除率升高(P＜0.05);病理切片中肾小管上皮细胞损伤减轻;肾小管上皮细胞凋亡率降低(P＜0.05).血清TNF-α和IFN-γ水平降低(P＜0.05).结论 ATRA通过抑制炎症反应和细胞凋亡,发挥保护缺血再灌注肾损伤的作用.%Objective To study the protective effect of total trans retinoic acid (ATRA) on renal injury induced by ischemia-reperfusion in rats.Methods 24 Sprague Dawley (SD) rats were divided into 4 groups of (1) Normal control group,(2) Sham operation group,(3) Model group:the model of renal ischemia-reperfusion was established.(4) Retinoic acid treatment Group:ATRA were given by gavage for 7 consecutive days.The rats were killed 24 hours after the model,and the serum and renal tissue were collected.Serum creatinine,tumor necrosis factor-α (TNF-αx) and interferon-γ (IFN-γ) levels,kidney biopsy,renal tubular score,renal damage and renal tissue apoptosis were detected.Results Compared with the model group,the serum creatinine level decreased and the creatinine clearance rate increased in ATRA treated group (P ＜ 0.05);the damage of renal tubular epithelial cells was alleviated in the pathological slices;and the apoptosis rate of renal tubular epithelial cells was decreased (P ＜ 0.05).Serum levels of TNF-α and IFN-γ decreased (P ＜ 0.05).Conclusions ATRA can protect the renal ischemia-reperfusion injury through inhibiting inflammatory reaction and apoptosis.