Objective To investigate the effects and mechanism of different doses of rosuvastatin on expression of tissue factor(TF) in cultured human monocyte-macrophage cells which were induced by oxidized low density lipoprotein (ox-LDL).Methods The human monocyte-macrophage cells were divided into seven groups:control group,ox-LDL group,poly-insine monophosphate group,different doses of rosuvastatin group(0.01 μmol/L,0.1 μmol/L,1 μmol/L,5 μmol/L).The expression of LOX-1 mRNA and TF mRNA was assayed by RT-PCR.The enzyme-linked immunosorbent assay was performed to determine the protein concentration of TF.Results Effects of different doses of rosuvastatin on expressions of LOX-1mRNA,TF mRNA and TF in cultured human monocyte-macrophage cells induced by ox-LDL:comparison among seven groups,the difference was statistically significant (F =91.334,58.833,103.552,P ＜0.05).Compared with control group,the expressions of LOX-1 mRNA,TF mRNA and TF were increased in the ox-LDL group[(3.25156 ± 0.15772) vs (1 ±0) ;(2.522451 ±0.138967) vs (1 ±0) ;(207.7233± 1.154701)ng/L vs (184.8467 ± 0.871799)ng/L],and they were in a concentration-dependent manner (P ＜ 0.05).Compared with the PolyⅠ group and the different doses of rosuvastatin group,the expressions of LOX-1 mRNA,TF mRNA and TF were in the ox-LDL group,and the different doses of rosuvastatin were decreased by dose-dependent manner.It was in a concentration dependent manner (P ＜ 0.05).Different doses of rosuvastatin were compared between groups (between each group P ＜ 0.05),the difference between each two groups was statistically significant (P ＜ 0.05).Conclusions LOX-1 may be responsible for the expression of TF in Human monocyte-macrophage cells induced by ox-LDL.Rosuvastatin by dose dependent manner and by means of ox-LDL reduced monocyte-macrophage LOX-1 mRNA and TF mRNA expressions,which reduced expression of TF.%目的 研究不同剂量瑞舒伐他汀对氧化修饰低密度脂蛋白(ox-LDL)诱导的人单核-巨噬细胞组织因子(TF)表达的影响及可能机制.方法 实验分七组:空白对照组、ox-LDL对照组、多聚肌苷酸组、不同剂量(0.01、0.1、1、5μmol/L)瑞舒伐他汀组.RT-PCR检测各组血凝素样氧化低密度脂蛋白受体-1(LOX-1)mRNA、TF mRNA的表达水平,ELISA检测各组TF蛋白含量的表达.结果 不同剂量瑞舒伐他汀对ox-LDL诱导后人单核-巨噬细胞LOX-1 mRNA、TF mRNA和TF表达的影响:七组之间比较,差异均有统计学意义(F =91.334,58.833,103.552,P＜0.05).ox-LDL组与空白组比较,LOX-1 mRNA、TF mRNA、TF的表达均增加[(3.25156±0.15772) vs(1±0)；(2.522451±0.138967) vs(1±0)；(207.7233±1.154701)ng/L vs(184.8467±0.871799) ng/L],差异有统计学意义(P＜0.05)；多聚肌苷酸组、各瑞舒伐他汀组与ox-LDL组比较,三者表达均下降,且不同剂量瑞舒伐他汀组表达呈剂量依赖性减少,差异均有统计学意义(P＜0.05)；不同剂量瑞舒伐他汀组之间进行两两比较时,各组间差异有统计学意义(均P ＜0.05).结论 LOX-1介导的ox-LDL可使人单核-巨噬细胞TF表达增加,瑞舒伐他汀可通过ox-LDL途径,且呈剂量依赖性下调单核-巨噬细胞LOX-1 mRNA、TFmRNA的表达,使TF蛋白表达减少.
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