METHOD OF OBTAINING CELLS OF MONOCYTE ORIGIN INDUCING TRANSPLANT ACCEPTANCE, METHOD OF OBTAINING PHARMACEUTICAL COMPOSITION SUPPRESSING TRANSPLANT REJECTION REACTION, CELLS OF MONOCYTE ORIGIN INDUCING TRANSPLANT ACCEPTANCE, CELL MEDICINE FOR TRANSPLANT ACCEPTANCE INDUCTION, PHARMACEUTICAL COMPOSITION SUPPRESSING TRANSPLANT REJECTION REACTION, APPLICATION OF CELLS INDUCING TRANSPLANT ACCEPTANCE (VERSIONS),METHOD OF OBTAINING AND/OR REPRODUCTION OF REGULATORY T-LYMPHOCYTE, HYBRIDOMA CELL LINE, ANTIBODY AND ANTIBODY APPLICATION

摘要

FIELD: medicine.;SUBSTANCE: invention concerns obtainment of cells of monocyte origin, inducing transplant acceptance, expressing antigens CD3 and CD14, and can be applied in transplantology. Monocytes are extracted from blood, reproduced in cultural medium with 1-20 mcg/l of cell growth factor M-CSF and cultivated for 24-72 hours in cultural medium with 0.1-20 ng/ml of interferon γ-IFN. Further the cells are extracted from cultural medium. For cell CD3 and CD14 expression antibodies produced by hybridoma DSM ACC2542 are used. If necessary, obtained cells are suspended and freezed.;EFFECT: obtainment of cells of monocyte origin, inducing transplant acceptance and suppressing transplant rejection reaction.;26 cl, 29 dwg, 1 tbl, 13 ex