慢性牙周炎和慢性牙周炎合并慢性阻塞性肺疾病患者血清中IL-2、IL-4、IL-6、IL-10、IFN-γ及TNF-α的检测及临床意义

摘要

目的 探讨慢性牙周炎和慢性牙周炎合并慢性阻塞性肺疾病(COPD)患者血清白细胞介素-2(IL-2)、IL4、IL-6、IL-10、干扰素-γ(IFN-γ)、肿瘤坏死因子-α(TNF-α)的表达水平及临床意义.方法 收集2015年8月至2016年8月朝阳医院31例慢性牙周炎合并COPD患者设为A组、31例慢性牙周炎患者设为B组,另收集同期31例单纯COPD患者设为对照组.抽取各组患者空腹静脉血4 ml,以细胞因子微球检测技术(CBA)测定血清炎性因子指标(IL-2、IL4、IL-6、IL-10、IFN-γ、TNF-α)水平,并统计对比各组牙周情况[菌斑指数(PLI)、临床附着丧失(CAL)、探诊深度(PD)、出血指数(BI)]及肺功能指标[第1秒用力呼气容积占预计值百分比(FEV1％)、第1秒用力呼气容积占用力肺活量百分比(FEV1/FVC)].结果 B组PLI、CAL、PD、BI大于对照组,A组各指标大于B组,差异有统计学意义(P＜0.05).B组FEV1％、FEV1/FVC与对照组比较差异无统计学意义(P＞0.05),A组各指标小于B组、对照组,差异有统计学意义(P＜0.05).B组血清IL-4、IL-6、IFN-γ、TNF-α水平高于对照组,A组各指标水平高于B组;B组血清IL-2、IL-10水平低于对照组,A组各指标水平低于B组,差异有统计学意义(P＜0.05).结论 慢性牙周炎及慢性牙周炎合并COPD患者牙周状况及肺功能状况不佳,血清IL-4、IL-6、IFN-γ、TNF-α水平增高及IL-2、IL-10水平降低可能为慢性牙周炎及COPD发病和进展的重要危险因素,可通过检测上述指标水平调整治疗方案.%Objective To explore the expression level and clinical significance of serum interleukin-2 (IL-2),IL-4,IL-6,IL-10,interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) in patients with chronic periodontitis and chronic periodontitis complicated with chronic obstructive pulmonary disease (COPD).Methods From August 2015-August 2016,31 COPD patients combined with chronic periodontitis were set as group A,31 patients with chronic periodontitis were set as group B,and another 31 healthy subjects were selected as the control group in Chaoyang Hospital.Each group extracted fasting venous blood 4 ml,serum inflammatory factors levels (IL-2,IL-4,IL-6,IL-10,IFN-γ and TNF-α) were measured by cytometric bead array (CBA),periodontal condition [plaque index (PLI),clinical attachment loss (CAL),probing depth (PD),bleeding index (BI)] and lung function index [first 1 s forced expiratory volume％ of predicted value (FEV1％),first 1 s forced expiratory volume occupies the percentage of vital capacity (FEV1/FVC)] were compared.Results The PLI,CAL,PD and BI levels in group B were higher than those in control group,indicators of group A were higher than group B,the difference was statistically significant (P ＜ 0.05).There was no significant difference in FEV1 ％ and FEV1/FVC between group B and control group,indicators of group A were less than group B and control group,the difference was statistically significant (P ＜ 0.05).The serum levels of IL-4,IL-6,IFN-γ and TNF-α in group B were higher than those in control group,indicators of group A were significantly higher than group B,the levels of IL-2 and IL-10 in group B were significantly lower than those in control group,indicators of group A were significantly less than group B (P ＜ 0.05).Conclusions Periodontal status and lung function of chronic periodontitis and chronic periodontitis with COPD patients is not good,the serum levels of IL-4,IL-6,IFN-γ,and TNF-α were increased,and the serum levels of IL-2 and IL-10 were decreased,which might be important risk factors for chronic periodontitis and COPD happen and progress.Therefore,treatment regimen can be adjusted by detecting the levels of above indicators.