白细胞介素6/代谢

摘要： 目的 探讨微切口超声乳化术对白内障患者的临床治疗效果及其对患者泪液IL-6、TNF-α、表皮生长因子(EGF)表达的影响.方法 采用前瞻性研究,将284例白内障患者按照随机数字表法分为对照组(采用同轴常规切口超声乳化术)和观察组(同轴微切口超声乳化术),每组142例,比较两组患者手术中有效超声乳化时间及平均超声能量差异,并分别于术前(T0)、术后1周(T1)、术后2周(T2)、术后4周(T3)对两组患者裸眼视力恢复情况,眼表疾病指数(OSDI)、泪膜破裂时间(BUT),基础泪液分泌试验(SIt),角膜荧光素染色(FL)以及泪液中IL-6、TNF-α、EGF表达水平进行检测,并对以上各指标进行Spearman相关性分析.结果 两组在有效超声乳化时间及平均超声能量上差异无统计学意义(P＞0.05),观察组裸眼视力改善程度优于对照组,差异有统计学意义(F=6.116,P=0.032);两组在不同时间点下,OSDI、FL、IL-6、TNF-α、EGF指标均呈现先升高后下降趋势,且观察组优于对照组,差异有统计学意义(P＜0.05);同时,BUT及SIt呈现先下降后升高趋势,且观察组优于对照组,差异有统计学意义(P＜0.05);泪液中IL-6、TNF-α、EGF表达量分别与OS-DI、FL评分呈正相关(P＞0.05).结论 微切口超声乳化术有助于降低白内障患者术后IL-6、TNF-α水平,提高EGF水平以及减少术后干眼等不良症状发生率,从而提高临床治疗效果.%Objective To investigate the effect of micro-incision phacoemul-sification on cataract patients and its effect on interleukin (IL)-6, tumor necrosis factor-α (TNF-α), and epidermal growth factor (EGF) expressions in cataract patients.Methods With forward-looking research, 284 cataract patients were randomly included in the control (coaxial conventional incision phacoemulsification) or observation (coaxial micro-incision phacoemulsification) groups, with 142 cases per group.The effective phacoemulsification time and mean ultrasonic energy difference were compared between two groups, and the uncorrected vision recovery, Ocular Surface Disease Index (OSDI), tear break-up time (BUT), schirmer I test (SIt), corneal fluorescence stain (FL), and the expressionsIL-6, TNF-α and EGF were measured in two groups on preoperative (T0), 1 week after operation (T1), 2 weeks after operation (T2), 4 weeks after operation (T3), and the above indicators of Spearman correlation analysis.Results There was no significant difference in effective phacoemulsification time and mean ultrasonic energy between two groups (P ＞ 0.05).The improvement of uncorrected visual acuity in observation group was significantly better than that in the control group (F=6.116, P =0.032).In addition, OSDI, FL,IL-6,TNF-α, and EGF showed the first increase and then decreased at different time points, and the observation group was superior to the control group with statistically significant difference.At the same time, BUT and SIt showed a trend of increasing first and then decreased, and the observation group was superior to the control group with statistically significant difference.In addition, IL-6, TNF-α, and EGF expressions were positively correlated with OSDI and FL scores (P ＞ 0.05).Conclusions Micro-incision phacoemulsification can help reduce the expressions of IL-6 and TNF-α, improve the expression levels of EGF in cataract patients, and reduce the incidence of adverse symptoms such as dry eye after operation.It might improve the clinical therapeutic effect.

摘要： Objective The plasma levels of interleukin 6 (IL-6),high sensitive C reactive protein (hs-CRP) and the level of T cell activation were detected in the peripheral blood inflammatory factors of acquired immunodeficiency syndrome (AIDS) patients,and the relationship with opportunistic infection and prognosis was analyzed.Methods 79 human immunodeficiency virus (HIV)-positive/aids cases from May 2014 to January 2015 in first hospital of Changsha were enrolled in the study.They were divided into three groups:HIV-infected without opportunistic infections group (n =20),HIV-infected with infections group (n =43,including HIV-infected with tuberculosis group,HIV-infected with merge fungus group.HIV combined hepatitis C),death group (n =16).Serum IL-6 and the concentration of hs-CRP were detected by enzyme-linked immunosorbent assay (ELISA).Flow cytometry was used to test the CD3 + CD4 + cell count,the percentage of CD4 + CD38 + cell and CD8 + CD38 + cell in peripheral blood mononuclear cell (PBMC).Compare the differences among the three groups.Results The results showed that:the concentration of hsCRP and IL-6 in peripheral blood of HIV death group was significantly higher than that in other three groups (P ＜ 0.05).The concentration of hs-CRP and IL-6 in the peripheral blood of the HIV-infected with tuberculosis group and HIV-infected with merge fungus group were significantly higher than that in the HIV-infected without opportunistic infections group (P ＜ 0.05),and the hs-CRP in the peripheral blood of the HIV combined with the hepatitis C group was higher than that in the HIV-infected without opportunistic infections group (P ＜ 0.05).The number of CD3,CD4T lymph nodes in the death group and the combined opportunistic infection group was significantly lower than that in the HIV-infected without opportunistic infections group (P ＜ 0.05).The HIV-RNA expression in peripheral blood of the death group and the combined opportunistic infection group was significantly lower than that in the HIV-infected without opportunistic infections group (P ＜ 0.05).The expression of CD8+CD38+ on PBMC in the HIV-infected without opportunistic infections group was significantly lower than that in the death group,the tuberculosis group,the fungus group and the hepatitis C group (P ＜ 0.05).The expression of CD4+ CD38 + on PBMC in the HIV-infected without opportunistic infections group was higher than that in the death group (P ＜ 0,05).Conclusions The concentration of inflammatory cytokines (IL-6,hs-CRP) and the expression level of T cell surface CD8 + CD38 + related to immune activation were associated with opportunistic infection and prognosis of AIDS.%目的 检测艾滋病患者外周血炎性因子血浆白细胞介素6(IL-6)、高敏C-反应蛋白(hs-CRP)的浓度和T细胞激活水平,分析其与机会性感染及预后的关系.方法 将2014年5月至2015年1月在长沙市第一医院就诊的79例HIV/AIDS患者纳入本研究,分为HIV无机会性感染组20例,HIV合并感染组43例(包括结核组16例、HIV合并丙肝组12例、HIV合并马尔尼菲青霉菌组15例),HIV死亡组16例.检测IL-6、hs-CRP的浓度;外周血PBMC中CD4+ CD38+细胞亚群和CD8+ CD38+细胞亚群的百分比,比较各组的差异.结果 HIV死亡组患者外周血hs-CRP、IL-6浓度明显高于无机会性感染组、HIV合并感染组,差异有统计学意义(P＜0.05).HIV合并感染组中的结核组、真菌感染组外周血hs-CRP、IL-6浓度明显高于无机会性感染组(P＜0.05),HIV合并丙肝组外周血hs-CRP高于无机会性感染组(P＜0.05).死亡组、各合并机会性感染组的CD3+CD4+T淋巴计数明显低于无机会性感染组(P＜0.05),死亡组、各合并机会性感染组的外周血HIV-RNA明显低于无机会性感染组(P＜0.05).无机会性感染组PBMC上CD8+ CD38+表达明显低于死亡组、结核组、真菌组、丙肝组(P＜0.05).无机会性感染组PBMC上CD4+ CD38+表达高于死亡组(P＜0.05).结论 与T细胞激活相关的细胞因子hs-CRP、IL-6及T细胞表面标志物CD8+ CD38+双表达的细胞百分比可能与艾滋病的机会性感染及预后有关.

摘要： 目的 探讨术前新辅助化疗对非小细胞肺癌疗效及围手术期并发症的影响.方法 将182例非小细胞肺癌患者按随机数字表法分为对照组和观察组各91例,对照组的患者术前未进行化疗直接采用手术治疗,观察组患者给予MVP方案化疗2个周期后采用手术治疗.观察麻醉诱导前(T0)、开胸后2 h(T1)、术毕(T2)、术后6 h(T3)和术后24 h(T4)血清肿瘤坏死因子-α(TNF-α)与白细胞介素-6(IL-6)水平、手术时间、术中出血量、住院时间、围手术期并发症及1、2、3年生存率.结果 两组T1、T2、T3、T4时TNF-α和IL-6与T0时比较均显著升高(P＜0.05),且观察组与同期对照组比较TNF-α和IL-6水平均显著升高(P＜0.05).观察组手术时间短于对照组,差异有统计学意义(P＜0.05);两组术中出血量和住院时间比较差异均无统计学意义(P＞0.05).观察组围手术期并发症发生率显著高丁对照组(P＜0.05).观察组1、2年生存率与对照组比较差异无统计学意义(P＞0.05),3年生存率显著高于对照组,且差异有统计学意义(P＜0.05).结论 新辅助化疗可改善非小细胞肺癌患者生存率,但亦会增加患者手术过程中炎症反应和围手术期并发症,临床应用新辅助化疗时应考虑其增加炎性反应的问题.

摘要： 目的 探讨米非司酮治疗子宫内膜异位症患者对其腹腔液血管内皮生长因子(VEGF)、白细胞介素6(IL-6)、糖链抗原199(CA199)表达的影响.方法 选取2013年1月至2016年1月本院妇产科收治的270例子宫内膜异位症患者为研究对象,按照随机数字表法分组原则,将其分为观察组与对照组,每组135例.对照组给予口服孕三烯酮治疗,观察组给予米非司酮治疗;治疗3个月后比较两组临床疗效,包括治疗前、治疗后1个月、治疗后3个月患者卵巢功能、腹腔液中VEGF、IL-6、CA199水平及不良反应发生情况.结果 观察组总有效率为90.37％,高于对照组总有效率88.15％,但差异无统计学意义(P＞O.05).治疗前,观察组与对照组卵泡刺激素(FSH)、雌二醇(E2)、黄体生成索(LH)水平比较,差异无统计学意义(P＞0.05);治疗后1、3个月,观察组FSH、E2、LH水平变化幅度小于对照组,差异有统计学意义(P＜0.05).治疗前,观察组与对照组VEGF、IL-6、CA199水平比较,差异无统计学意义(P＞0.05);治疗后1、3个月,观察组与对照组VEGF、IL-6、CA199水平均明显低于治疗前,差异有统计学意义(P＜0.05),且观察组VEGF、IL-6、CA199水平明显低于对照组(P＜0.05);观察组不良反应发生率明显低于对照组(P＜0.05).结论 米非司酮可明显降低子宫内膜异位症患者腹腔液VEGF、IL-6 、CA199水平,对患者卵巢功能具有积极影响,有较高临床应用价值.

摘要： 目的:探讨米力农对老年心力衰竭患者心功能及血清hs-CRP、TNF-α、IL-6水平的影响.方法:将72例老年慢性心力衰竭患者随机分为观察组(n=36)与对照组(n=36),对照组给予常规抗心衰治疗,观察组患者联合应用米力农治疗,比较两组患者临床疗效及治疗前后心功能指标(超声心动图、血清NT-pro BNP)及血清hs-CRP、TNF-α、IL-6水平的变化.结果:治疗后,观察组治疗总有效率为91.67%,较对照组(75.00%)明显升高,差异有有统计学意义(P<0.05);与治疗前比较,LVEF在两组患者均获得了明显升高 LVEDd及血清NT-pro BNP水平明显下降,观察组患者上述指标均优于对照组患者(P<0.05);治疗后,血清hs-CRP、TNF-α及IL-6水平在两组患者均降低明显,且观察组上述指标明显低于对照组,差异均有统计学意义(P<0.05).结论:短期使用米力农治疗老年慢性心力衰竭患者,可以明显改善患者的心功能、抑制炎症反应、提高临床疗效.%Objective:To investigate the effect of milrinone on cardiac function in elderly patients with heart failure and its impact on serum hs-CRP, TNF-α and IL-6.Methods:72 cases of elderly patients with chronic heart failure were randomly divided into observation group (n=36) and control group (n=36), the control group received routine anti heart failure treatment, observation of combined application of milrinone in the treatment group, compared two groups of patients with clinical curative effect, cardiac function index before and after treatment between the two groups (echocardiography, serum NT-pro BNP) index, serum NT-pro changes and serum hs-CRP, TNF-alpha, IL-6 level.Results:after treatment, the total efficiency of treatment in the observation group was 91.67%, compared with the control group (75%) was significantly increased, there was significant difference (P<0.05);compared with before treatment, LVEF in the two groups of patients were significantly increased LVEDd and serum NT-pro BNP levels were significantly decreased, the observation group of patients with these indexes better than the control group (P<0.05);after treatment, serum hs-CRP, TNF-alpha and IL-6 levels in the two groups of patients were significantly decreased, and the observation group were lower than the control group, there were significant differences (P<0.05).Conclusion:For the short-term use of milrinone in the treatment of elderly patients with chronic heart failure can significantly improve cardiac function and inhibit inflammatory reaction and improve the clinical efficacy.

摘要： 目的:探讨冠心病患者血浆血红素加氧酶-1(HO-1)、白细胞介素-6(IL-6)和内脂素水平与冠状动脉病变严重程度的相关性.方法:选择胸痛患者217例,依据冠脉造影结果分为单支病变组、双支病变组、多支病变组和冠脉正常组;采用Gensini积分评价冠状动脉病变严重程度,应用酶联免疫吸附法测定血浆HO-1、IL-6及内脂素.结果:血浆HO-1水平冠脉病变组较正常组显著降低,并且三支血管病变组较双支血管病变及单支血管病变组显著降低(P<0.05);而血浆IL-6及内脂素水平冠脉病变组较正常组显著升高,并且三支血管病变组较双支血管病变及单支血管病变组显著升高(P<0.05).Gensini积分≥30分组血浆HO-1水平明显低于<30 分组(P<0.05),而IL-6及内脂素水平则明显升高(P<0.05).相关分析显示:Gensini积分分别与IL-6及内脂素正相关(r=0.583,P<0.01;r=0.724,P<0.01),与血浆HO-1水平负相关(r=-0.517,P<0.01);HO-1分别与IL-6及内脂素负相关(r=-0.614,P<0.01;r=-0.517,P<0.01),而IL-6与内脂素正相关(r=0.648,P<0.01).结论:血浆HO-1、IL-6及内脂素参与冠心病的发病过程,并与冠状动脉病变严重程度密切相关.

摘要： Objective :To investigate the effects of ulinastatin (UTI) for traumatic brain injury (TBI) treat‐ment and effect on serum index . Methods :120 TBI patients were selected and divided into control group and ob‐servation group .60 cases were in each group .All patients were given TBI routine treatment .On this basis ,the ob‐servation group was added with UTI static push .The serological indexes and therapeutic effect of the two groups were compared . Results :The serum levels of TNF‐6 ,S‐100B and CRP in the two groups at 7d and 14d after treat‐ment were significantly lower than those before treatment (P< 0 .05) .The change of observation group was signifi‐cantly higher than that of control group (P< 0 .05) .Compared with before treatment ,the two groups of treatment 14d ,GCS 7d scores were significantly increased (P< 0 .05) ,while the observation group increased more significant‐ly ,and was different from the control group significantly (P < 0 .05 ) .The total effective rate of the observation group was significantly higher than that of the control group (78 .33% vs .55% ,P< 0 .05) . Conclusion :Ulinasta‐tin can effectively improve the therapeutic effect in patients with TBI ,alleviate the inflammatory reaction and protect nerve function .%目的：探讨乌司他丁（U TI）治疗颅脑外伤（TBI）患者的治疗效果及对血清学指标的影响。方法：将120例 TBI 患者随机分为观察组和对照组，两组患者均给予 TBI 常规治疗，观察组在此基础上加用 U TI 静推。检测两组患者的血清学指标，观察比较其治疗效果。结果：治疗后第7、14天，两组患者血清 IL‐6、TNF‐α、CRP 、S‐100B 和 NSE 水平均较治疗前明显下降（P＜0．05），观察组下降幅度更大，与对照组比较差异有显著性（P＜0．05）；与治疗前相比，两组治疗第7、14天 GCS评分均显著上升（P＜0．05），而观察组上升更明显，与对照组差异显著（P＜0．05）；观察组治疗总有效率显著高于对照组（78．33％与55．00％，P ＜0．05）。结论：乌司他丁可有效提高 TBI 患者治疗效果、缓解炎性反应、保护神经功能。

摘要： 目的 探讨慢性牙周炎和慢性牙周炎合并慢性阻塞性肺疾病(COPD)患者血清白细胞介素-2(IL-2)、IL4、IL-6、IL-10、干扰素-γ(IFN-γ)、肿瘤坏死因子-α(TNF-α)的表达水平及临床意义.方法 收集2015年8月至2016年8月朝阳医院31例慢性牙周炎合并COPD患者设为A组、31例慢性牙周炎患者设为B组,另收集同期31例单纯COPD患者设为对照组.抽取各组患者空腹静脉血4 ml,以细胞因子微球检测技术(CBA)测定血清炎性因子指标(IL-2、IL4、IL-6、IL-10、IFN-γ、TNF-α)水平,并统计对比各组牙周情况[菌斑指数(PLI)、临床附着丧失(CAL)、探诊深度(PD)、出血指数(BI)]及肺功能指标[第1秒用力呼气容积占预计值百分比(FEV1％)、第1秒用力呼气容积占用力肺活量百分比(FEV1/FVC)].结果 B组PLI、CAL、PD、BI大于对照组,A组各指标大于B组,差异有统计学意义(P＜0.05).B组FEV1％、FEV1/FVC与对照组比较差异无统计学意义(P＞0.05),A组各指标小于B组、对照组,差异有统计学意义(P＜0.05).B组血清IL-4、IL-6、IFN-γ、TNF-α水平高于对照组,A组各指标水平高于B组;B组血清IL-2、IL-10水平低于对照组,A组各指标水平低于B组,差异有统计学意义(P＜0.05).结论 慢性牙周炎及慢性牙周炎合并COPD患者牙周状况及肺功能状况不佳,血清IL-4、IL-6、IFN-γ、TNF-α水平增高及IL-2、IL-10水平降低可能为慢性牙周炎及COPD发病和进展的重要危险因素,可通过检测上述指标水平调整治疗方案.%Objective To explore the expression level and clinical significance of serum interleukin-2 (IL-2),IL-4,IL-6,IL-10,interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) in patients with chronic periodontitis and chronic periodontitis complicated with chronic obstructive pulmonary disease (COPD).Methods From August 2015-August 2016,31 COPD patients combined with chronic periodontitis were set as group A,31 patients with chronic periodontitis were set as group B,and another 31 healthy subjects were selected as the control group in Chaoyang Hospital.Each group extracted fasting venous blood 4 ml,serum inflammatory factors levels (IL-2,IL-4,IL-6,IL-10,IFN-γ and TNF-α) were measured by cytometric bead array (CBA),periodontal condition [plaque index (PLI),clinical attachment loss (CAL),probing depth (PD),bleeding index (BI)] and lung function index [first 1 s forced expiratory volume％ of predicted value (FEV1％),first 1 s forced expiratory volume occupies the percentage of vital capacity (FEV1/FVC)] were compared.Results The PLI,CAL,PD and BI levels in group B were higher than those in control group,indicators of group A were higher than group B,the difference was statistically significant (P ＜ 0.05).There was no significant difference in FEV1 ％ and FEV1/FVC between group B and control group,indicators of group A were less than group B and control group,the difference was statistically significant (P ＜ 0.05).The serum levels of IL-4,IL-6,IFN-γ and TNF-α in group B were higher than those in control group,indicators of group A were significantly higher than group B,the levels of IL-2 and IL-10 in group B were significantly lower than those in control group,indicators of group A were significantly less than group B (P ＜ 0.05).Conclusions Periodontal status and lung function of chronic periodontitis and chronic periodontitis with COPD patients is not good,the serum levels of IL-4,IL-6,IFN-γ,and TNF-α were increased,and the serum levels of IL-2 and IL-10 were decreased,which might be important risk factors for chronic periodontitis and COPD happen and progress.Therefore,treatment regimen can be adjusted by detecting the levels of above indicators.

摘要： 目的 探讨妊娠期肝内胆汁淤积症(ICP)孕妇血清白细胞介素6(IL-6)、白细胞介素10(IL-10)、肿瘤坏死因子α(TNF-α)水平对母婴结局的影响.方法 选取2015年1月至2017年1月本院收治的126例ICP孕妇患者为研究对象,其中轻度ICP者52例,重度ICP者74例,同期纳入48例乙型肝炎女性为肝炎对照组,50例正常体检健康孕妇作为对照组,采用酶联免疫吸附法(ELISA)检测受试者血清IL-6、IL-10、TNF-α水平,分析三者水平与母婴结局之间的关系.结果 肝炎组IL-6、TNF-α水平明显高于ICP组与对照组,IL-10水平明显低于ICP组与对照组(P＜0.05),ICP组IL-6、TNF-α水平明显高于对照组(P＜0.05),IL-10水平明显低于对照组(P＜0.05);不同分级ICP组比较,重度ICP组血清IL-6、TNF-α水平均明显高于轻度ICP组(P＜0.05),IL-10水平明显低于轻度ICP组(P ＜0.05);ICP组产妇羊水污染、剖宫产、早产、产后出血、胎儿窘迫、早产低体重儿、新生儿窒息发生率明显高于对照组(P＜0.05);重度ICP组产妇羊水污染、剖宫产、早产、产后出血、胎儿窘迫、早产低体重儿、新生儿窒息发生率明显高于轻度ICP组(P ＜0.05);ICP组中不良妊娠结局者血清IL-6、TNF-α水平明显高于正常妊娠结局者(P＜0.05),IL-10水平明显低于正常妊娠结局者(P＜0.05).结论 IL-6、IL-10、TNF-α水平与ICP病情严重程度以及母婴结局有关,推测三者对于母婴结局预测具有重要参考价值.

摘要： 目的 探讨采用腹腔注射吸烟提取物(CSE)构建的慢性阻塞性肺疾病(COPD)小鼠模型肺组织中蛋白精氨酸甲基转移酶6(PMRT6)的表达及其与IL-6和COX-2基因表达的关系.方法 16只雄性6周龄近交无特定病原体(SPF)级C57BL/8J小鼠随机分为正常对照组(8只)和COPD组(8只),采用经腹腔注射CSE方法建立小鼠COPD模型.检测小鼠肺功能并收集两组小鼠肺组织,HE染色检测小鼠肺组织病理改变,Western blotting检测两组小鼠肺组织中PRMT6蛋白表达情况及组蛋白H3R2进行非对称双甲基化(H3R2me2a)和H3K4的三甲基化(H3 K4me3)信号水平,qRTPCR检测小鼠肺组织中PMRT6及IL-6、COX-2的mRNA表达情况.结果 与对照组比较,COPD模型小鼠肺组织呈现典型肺气肿改变,肺功能显著下降,并且肺组织中PRMT6的mRNA及蛋白表达显著下降,组蛋白H3R2me2a信号水平下调,而H3K4me3信号水平上调,同时IL-6和COX-2的mRNA表达均增高,PRMT6与IL-6、COX-2的mRNA表达呈负相关.结论 CSE诱导的COPD小鼠模型中PRMT6明显下调,抑制组蛋白H3R2二甲基化并促进H3 K4三甲基化,PRMT6与IL-6和COX-2炎症基因转录表达呈负相关,PRMT6可能通过调控组蛋白甲基化水平激活炎症基因的转录表达参与COPD的发生.%Objective To explore the expression of protein arginine methyltransferases 6 (PRMT6) in chronic obstructive pulmonary disease (COPD) mouse model and its correlation with inflam mation gene interleukin 6 (IL-6) and cyclooxygenase 2 (COX-2).Methods Sixteen C57BL/8J mice were randomly divided into 2 groups:control group and cigarette smoke extract (CSE)-induced COPD group.Each group was injected intraperitoneally with phosphate-buffered saline solutions (PBS) or CSE at days 1,12,23 and measured lung function and collected lung tissue at day 29.The morphology change of the lung tissue was determined by hematoxylin and eosin (HE) stainning.The protein expression of PRMT6,H3R2me2a and H3K4me3 were detected in lung homogenates by Western-blotting.The mRNA expression of PRMT6,IL-6 and COX-2 were measured by quantitative real-time polymerase chain reaction (qRT-PCR).Results Comparing to control group,COPD group showed typical emphysema changes in the lung tissue,and significantly decreased lung function.The mRNA and protein expression of PRMT6 in the lung tissue of the mice with COPD were significantly decreased,following with the down-regulated signal level of H3R2me2a protein expression,while the increased level of IL-6 and COX-2 mRNA.Meanwhile,PRMT6 was negatively correlated with IL-6 and COX-2 mRNA expression.Conclusions PRMT6 was significantly reduced in CSE-induced COPD mouse model,following with decreased histone H3R2 dimethylation and increased H3K4 trimethylation,negatively correlating with inflammatory gene IL-6 and COX-2 transcription expression.PRMT6 downregulation may activate the transcriptional expression of inflammatory genes involved in the development of COPD,through the regulation of histone methylation level.

摘要： 本发明涉及免疫分析领域，特别涉及一种检测白细胞介素‑6的试剂盒以及非诊断目的检测白细胞介素‑6的方法。该试剂盒包括生物素化抗体、酶标记抗体、化学发光底物和亲和素偶联的超顺磁微粒；生物素化抗体为生物素N羟基丁二酰亚胺酯与抗白细胞介素‑6单克隆抗体A偶联的产物；酶标记抗体为酶与抗白细胞介素‑6单克隆抗体B偶联的产物。本发明提供的试剂盒具有检测灵敏度高，特异性好，检测时间短，检测过程简单，并能方便用于全自动检测仪器等优点。

摘要： 本发明提供了完全人类单克隆抗体，所述的单克隆抗体能够识别所述的白细胞介素-6(IL-6)/白细胞介素-6受体(IL-6R)复合体。所述的发明进一步的提供了使用这样的单克隆抗体作为一种治疗剂、诊断剂、以及预防剂的方法。

摘要： 本发明提供筛选由白细胞介素6、白细胞介素13、TNF、G‑CSF、CXCL1、CXCL2、或CXCL5所引起的疾病的预防或治疗剂的方法，及由白细胞介素6、白细胞介素13、TNF、G‑CSF、CXCL1、CXCL2、或CXCL5所引起的疾病的预防或治疗剂。筛选由白细胞介素6、白细胞介素13、TNF、G‑CSF、CXCL1、CXCL2、或CXCL5所引起的疾病的预防或治疗剂的方法，所述方法以选自由MEX3B基因或MEX3B蛋白质的表达变化、及MEX3B蛋白质的功能变化组成的组中的至少一种作为指标。

摘要： 一种白细胞介素2(IL－2)－白细胞介素6(IL－6)－白细胞介素23(IL－23)三聚体蛋白，提供一种生物技术来制备该三聚体蛋白及其在高效抗肿瘤方面的应用。通过RT－PCR合成IL－2、IL－6及IL－23 cDNA，经过纯化扩增酶切，将两者连接重组至真核表达载体pcDNA3.1(+)。该重组质粒的表达产物为IL－2－IL－6－IL－23三聚体蛋白。其包含的三种细胞因子有较好的协同效应，抗肿瘤作用显著增强。

摘要： 本发明公开了一种使用具有白细胞介素-12受体的细胞检测重组人白细胞介素-12体外活性的方法及应用，包括以下步骤：（1）从液氮罐取出一支冻存的具有白细胞介素-12受体的细胞，复苏活化培养至对数生长期；（2）使用不同浓度的重组人白细胞介素-12标准品诱导具有白细胞介素-12受体的细胞产生γ干扰素，得到重组人白细胞介素-12标准品浓度值ED50s；（3）使用不同浓度的待测重组人白细胞介素-12蛋白质诱导具有白细胞介素-12受体的细胞产生γ干扰素，得到待测重组人白细胞介素-12蛋白质浓度值ED50r；（4）根据公式Pr=Ps×ED50s/ED50r，得到待测重组人白细胞介素-12蛋白质体外活性Pr。

摘要： 本申请公开了提供由宿主细胞共表达白细胞介素(IL)‑21和IL‑15的核酸和多肽，每种白细胞介素通过细胞膜锚定部分与细胞膜结合。还公开了相关的重组表达载体、宿主细胞、细胞群体、药物组合物以及治疗或预防癌症的方法。

摘要： 本发明涉及包括白细胞介素10和白细胞介素4的融合蛋白、编码这种融合蛋白的核酸分子、包含这种核酸分子的载体、及包含这种核酸分子或这种载体的宿主细胞。本发明进一步涉及用于产生这种融合蛋白的方法。所述融合蛋白或编码该融合蛋白的基因治疗载体可用于预防和/或治疗骨关节炎、慢性疼痛、以局部或全身炎症、免疫激活和/或淋巴组织增生为特征的病症。

摘要： 本发明涉及包含白细胞介素10和白细胞介素4的融合蛋白、编码这种融合蛋白的核酸分子、包含这种核酸分子的载体、及包含这种核酸分子或这种载体的宿主细胞。本发明进一步涉及用于产生这种融合蛋白的方法。所述融合蛋白或编码该融合蛋白的基因治疗载体可用于预防和/或治疗骨关节炎、慢性疼痛、以局部或全身炎症、免疫激活和/或淋巴组织增生为特征的病症。

摘要： 本发明涉及免疫分析领域，特别涉及一种检测白细胞介素-6的试剂盒以及非诊断目的检测白细胞介素-6的方法。该试剂盒包括生物素化抗体、酶标记抗体、化学发光底物和亲和素偶联的超顺磁微粒；生物素化抗体为生物素N羟基丁二酰亚胺酯与抗白细胞介素-6单克隆抗体A偶联的产物；酶标记抗体为酶与抗白细胞介素-6单克隆抗体B偶联的产物。本发明提供的试剂盒具有检测灵敏度高，特异性好，检测时间短，检测过程简单，并能方便用于全自动检测仪器等优点。

摘要： 本发明提供了完全人类单克隆抗体，所述的单克隆抗体能够识别所述的白细胞介素-6(IL-6)/白细胞介素-6受体(IL-6R)复合体。所述的发明进一步的提供了使用这样的单克隆抗体作为一种治疗剂、诊断剂、以及预防剂的方法。