苏氨酸双铜配合物对糖尿病小鼠血糖、血脂的影响及对心肾血管的保护作用

摘要

目的 探讨苏氨酸双铜配合物对糖尿病小鼠血糖、血脂的影响以及对心肾血管的保护作用.方法 采用四氧嘧啶腹腔注射制作小鼠糖尿病模型.灌胃给与糖尿病模型小鼠低、中、高(0.002%,0.02%,0.1%)三种剂量的苏氨酸双铜配合物,测定给药0,30及45天的空腹血糖,45天处死动物取血及心、肾组织,测定糖化血红蛋白、血脂及观察不同剂量药物对糖尿病小鼠的心、肾病理改变的影响.结果 与蒸馏水灌胃对照组比较:苏氨酸双铜配合物中剂量组降血糖作用最显著(P＜0.01),低剂量组降血糖作用次之(P＜0.05);苏氨酸双铜配合物低、中、高剂量组胆固醇(TC)水平明显降低(P＜0.05),高密度脂蛋白(HDL)水平明显升高(P＜0.05);中剂量组低密度脂蛋白(LDL)水平明显降低(P＜0.05);中剂量组心肌纤维排列整齐,细胞核和细胞浆分布均匀,肾小球结构正常,细胞大小分布均匀,染色正常.结论 苏氨酸双铜配合物具有显著的降血糖、调节血脂紊乱的作用,并对糖尿病小鼠的心肾组织起到明显的保护作用,其中以中剂量组(0.02%)效果最佳.%Aim To study the effects of binuclear copper (Ⅱ) threonine complex (Cu2 (Thr)4) as analogue of superoxide dismutase (SOD)on blood glucose, blood lipids and vessels of hearts and kidneys in diabetic mice. Methods Diabetic mouse model was established by intraperitioneal injection of alloxan. Low, middle, and high doses of Cu2(Thr)4 at 0.002%, 0.02% and 0.1% were given respectively to diabetic mice following lavage. The fasting blood glucose was determined after the diabetic mice were given Cu2 (Thr)4 for 0, 30, and 45 d. The diabetic mice were killed on the 45th day. Then glycosylated hemoglobin (HbA1c) and blood lipids were assayed, and pathologic changes in hearts and kidneys stained with HE were observed. Results Compared with the control group in which the diabetic mice were given distilled water, the value of blood glucose reduced significantly in middle dose group (P ＜ 0.01 ), followed by that in low dose group (P ＜ 0.05). TC level reduced markedly and HDL level increased significantly in all three treatment groups (P ＜ 0.05). Especially in middle dose group, cardiac muscle fibers were neatly arranged, nucleus and cytoplasm well distributed,glomeruli showing normal structure, cells well distributed and staining being normal. Conclusion Cu2 (Thr)4 reduces blood glucose,regulates blood lipids, and play protective action on the vessels of hearts and kidneys in diabetic mice. The effects of it in middle dose were better than those of other doses.